1α,25-dihydroxyvitamin D 3 -eluting nanofibrous dressings induce endogenous antimicrobial peptide expression.

Autor: Jiang J; Department of Surgery, Transplant & Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA., Zhang Y; Department of Biochemistry & Biophysics, Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.; Nutrition Graduate Program, School of Biological & Population Health Sciences, College of Public Health & Human Sciences, Oregon State University, Corvallis, OR 97331, USA., Indra AK; Department of Biochemistry & Biophysics, Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.; Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA.; Department of Dermatology, Oregon Health & Science University (OHSU), Portland, OR 97239, USA.; Knight Cancer Institute, OHSU, Portland, OR 97239, USA., Ganguli-Indra G; Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA.; Knight Cancer Institute, OHSU, Portland, OR 97239, USA., Le MN; Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA., Wang H; Department of Surgery, Transplant & Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA., Hollins RR; Department of Surgery - Plastic & Reconstructive Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA., Reilly DA; Department of Surgery - Plastic & Reconstructive Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA., Carlson MA; Department of Surgery - General Surgery & Genetics, Cell Biology & Anatomy, University of Nebraska Medical Center, Omaha, NE 68198, USA.; Department of Surgery, VA Nebraska - Western Iowa Health Care System, Omaha, NE 68105, USA., Gallo RL; Department of Dermatology, University of California, San Diego, CA 92093, USA., Gombart AF; Department of Biochemistry & Biophysics, Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.; Nutrition Graduate Program, School of Biological & Population Health Sciences, College of Public Health & Human Sciences, Oregon State University, Corvallis, OR 97331, USA., Xie J; Department of Surgery, Transplant & Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Jazyk: angličtina
Zdroj: Nanomedicine (London, England) [Nanomedicine (Lond)] 2018 Jun; Vol. 13 (12), pp. 1417-1432. Date of Electronic Publication: 2018 Jul 04.
DOI: 10.2217/nnm-2018-0011
Abstrakt: Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) and augmenting human CAMP induction.
Materials & Methods: Nanofibrous wound dressings containing 1,25(OH) 2 D 3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo.
Results: 1,25(OH) 2 D 3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH) 2 D 3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH) 2 D 3 -loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants.
Conclusion: 1,25(OH) 2 D 3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.
Databáze: MEDLINE
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