Chloroplast Acetyltransferase NSI Is Required for State Transitions in Arabidopsis thaliana .

Autor:	Koskela MM; Department of Biochemistry, Molecular Plant Biology, University of Turku, 20520 Turku, Finland., Brünje A; Plant Physiology, Institute of Plant Biology and Biotechnology, University of Muenster, 48149 Münster, Germany., Ivanauskaite A; Department of Biochemistry, Molecular Plant Biology, University of Turku, 20520 Turku, Finland., Grabsztunowicz M; Department of Biochemistry, Molecular Plant Biology, University of Turku, 20520 Turku, Finland., Lassowskat I; Plant Physiology, Institute of Plant Biology and Biotechnology, University of Muenster, 48149 Münster, Germany.; Plant Proteomics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Neumann U; Central Microscopy, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Dinh TV; Department of Plant Molecular Biology, Centre for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany., Sindlinger J; Interfaculty Institute of Biochemistry, University of Tübingen, 72076 Tübingen, Germany., Schwarzer D; Interfaculty Institute of Biochemistry, University of Tübingen, 72076 Tübingen, Germany., Wirtz M; Department of Plant Molecular Biology, Centre for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany., Tyystjärvi E; Department of Biochemistry, Molecular Plant Biology, University of Turku, 20520 Turku, Finland., Finkemeier I; Plant Physiology, Institute of Plant Biology and Biotechnology, University of Muenster, 48149 Münster, Germany pmulo@utu.fi iris.finkemeier@uni-muenster.de.; Plant Proteomics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Mulo P; Department of Biochemistry, Molecular Plant Biology, University of Turku, 20520 Turku, Finland pmulo@utu.fi iris.finkemeier@uni-muenster.de.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2018 Aug; Vol. 30 (8), pp. 1695-1709. Date of Electronic Publication: 2018 Jul 02.
DOI:	10.1105/tpc.18.00155
Abstrakt:	The amount of light energy received by the photosynthetic reaction centers photosystem II (PSII) and photosystem I (PSI) is balanced through state transitions. Reversible phosphorylation of a light-harvesting antenna trimer (L-LHCII) orchestrates the association between L-LHCII and the photosystems, thus adjusting the amount of excitation energy received by the reaction centers. In this study, we identified the enzyme NUCLEAR SHUTTLE INTERACTING (NSI; AT1G32070) as an active lysine acetyltransferase in the chloroplasts of Arabidopsis thaliana Intriguingly, nsi knockout mutant plants were defective in state transitions, even though they had a similar LHCII phosphorylation pattern as the wild type. Accordingly, nsi plants were not able to accumulate the PSI-LHCII state transition complex, even though the LHCII docking site of PSI and the overall amounts of photosynthetic protein complexes remained unchanged. Instead, the nsi mutants showed a decreased Lys acetylation status of specific photosynthetic proteins including PSI, PSII, and LHCII subunits. Our work demonstrates that the chloroplast acetyltransferase NSI is needed for the dynamic reorganization of thylakoid protein complexes during photosynthetic state transitions. (© 2018 American Society of Plant Biologists. All rights reserved.)
Databáze:	MEDLINE
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