Selective contrast agents with potential to the earlier detection of tumors: Insights on synthetic pathways, physicochemical properties and performance in MRI assays.

Autor: Montiel Schneider MG; INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Av. Alem 1253, Bahía Blanca, 8000, Argentina., Martin MJ; INBIOSUR, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur (UNS)-CONICET, San Juan 671, Bahía Blanca, 8000, Argentina., Coral DF; Instituto de Física La Plata IFLP-CONICET, Departamento de Física, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina; Institución Universitaria CESMAG, Pasto, Colombia., Muraca D; Laboratório de Materiais e Dispositivos, Instituto de Fïsica 'Gleb Wataghin', Universidade Estadual de Campinas, Campinas, Brazil., Gentili C; INBIOSUR, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur (UNS)-CONICET, San Juan 671, Bahía Blanca, 8000, Argentina., Fernández van Raap M; Instituto de Física La Plata IFLP-CONICET, Departamento de Física, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina., Lassalle VL; INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Av. Alem 1253, Bahía Blanca, 8000, Argentina. Electronic address: veronica.lassalle@uns.edu.ar.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2018 Oct 01; Vol. 170, pp. 470-478. Date of Electronic Publication: 2018 Jun 20.
DOI: 10.1016/j.colsurfb.2018.06.044
Abstrakt: Magnetic iron oxide nanoparticles (MNPs) have been prepared and stabilized with three organic acids (tartaric, malic and ascorbic) in order to obtain biocompatible and water dispersible MNPs with potential to bind specifically to tumoral cancer cells. An in deep characterization was performed aiming to verify the presence and effect of the coating and stabilizer on MNPs surface. Besides the mechanisms followed by the different acids to bind MNPs were elucidated and used to justify the differences in the physicochemical properties of each formulation. Data related to characterization revealed that MNPs coated with ascorbic acid (MNPs-AA) resulted the most suitable in terms of their size, surface charge and stability along the time. Besides, ascorbic acid may be recognized by GLUTs receptors that are overexpressed in several kinds of tumoral cells. Therefore, MNPs-AA was selected to explore its performance in both MRI and in vitro assays using human colon cancer cells HCT 116. MRI experiments were performed in clinical equipment using a series of aqueous dispersions of MNPs-AA that were evaluated as T 2 contrast agent. The T 2 - weighted images obtained as well as the calculated r2, indicated that MNPs-AA could act as efficient T 2 contrast agent for MRI. Regarding in vitro assays, MNPs-AA did not alter the cellular function neither exert cytotoxicity using the three explored doses. The internalization of the nanoparticles on the cellular structure was confirmed quanti and qualitatively using atomic absorption spectroscopy and Prussian blue techniques respectively. From these results, it emerges that ascorbic acid coated-magnetite nanoparticles may be used as alternative contrast agent to avoid or minimize some toxicological issues related to the widely used gadolinium.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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