C.B-17 SCID mice develop epicardial calcinosis with unaltered cardiac function.

Autor: Raghunathan S; Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, 77204, TX, USA., Reynolds CL; Mouse Phenotyping Core, Baylor College of Medicine, Houston, 77030, TX, USA., Schwartz RJ; Department of Biology and Biochemistry, University of Houston, Houston, 77204, TX, USA., Stewart MD; Department of Biology and Biochemistry, University of Houston, Houston, 77204, TX, USA., McConnell BK; Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, 77204, TX, USA.
Jazyk: angličtina
Zdroj: Fundamental & clinical pharmacology [Fundam Clin Pharmacol] 2019 Feb; Vol. 33 (1), pp. 25-30. Date of Electronic Publication: 2018 Aug 05.
DOI: 10.1111/fcp.12398
Abstrakt: Inbred mouse strains are the most widely used mammalian model organism in biomedical research owing to ease of genetic manipulation and short lifespan; however, each inbred strain possesses a unique repertoire of deleterious homozygous alleles that can make a specific strain more susceptible to a particular disease. In the current study, we report dystrophic cardiac calcinosis (DCC) in C.B-17 SCID male mice at 10 weeks of age with no significant change in cardiac function. Acquisition of DCC was characterized by myocardial injury, fibrosis, calcification, and necrosis of the tissue. At 10 weeks of age, 38% of the C.B-17 SCID mice from two different commercial colonies exhibited significant calcinosis on the ventricular epicardium, predominantly on the right ventricle. The frequency of calcinosis was more than 50% for mice obtained from Taconic's Cambridge City colony and 25% for mice obtained from Taconic's German Town colony. Interestingly, the DCC phenotype did not affect cardiac function at 10 weeks of age. No differences in echocardiography or electrocardiography were observed between the calcinotic and non-calcinotic mice from either colony. Our findings suggest that C.B-17 SCID mice exhibit DCC as early as 10 weeks of age with no significant impact on cardiac function. This strain of mice should be cautiously considered for the study of cardiac physiology.
(© 2018 Société Française de Pharmacologie et de Thérapeutique.)
Databáze: MEDLINE
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