CD4 + and CD8 + T-Cell-Specific DNA Cytosine Methylation Differences Associated With Obesity.

Autor: Hohos NM; Department of Foods and Nutrition, University of Georgia, Athens, Georgia, USA., Smith AK; Psychiatry and Behavioral Sciences, University of Emory School of Medicine, Atlanta, Georgia, USA., Kilaru V; Psychiatry and Behavioral Sciences, University of Emory School of Medicine, Atlanta, Georgia, USA., Park HJ; Department of Foods and Nutrition, University of Georgia, Athens, Georgia, USA., Hausman DB; Department of Foods and Nutrition, University of Georgia, Athens, Georgia, USA., Bailey LB; Department of Foods and Nutrition, University of Georgia, Athens, Georgia, USA., Lewis RD; Department of Foods and Nutrition, University of Georgia, Athens, Georgia, USA., Phillips BG; Clinical and Administrative Pharmacy, University of Georgia, Athens, Georgia, USA., Meagher RB; Department of Genetics, University of Georgia, Athens, Georgia, USA.
Jazyk: angličtina
Zdroj: Obesity (Silver Spring, Md.) [Obesity (Silver Spring)] 2018 Aug; Vol. 26 (8), pp. 1312-1321. Date of Electronic Publication: 2018 Jun 28.
DOI: 10.1002/oby.22225
Abstrakt: Objective: Lifestyle factors associated with obesity may alter epigenome-regulated gene expression. Most studies examining epigenetic changes in obesity have analyzed DNA 5´-methylcytosine (5mC) in whole blood, representing a weighted average of several distantly related and regulated leukocyte classes. To examine leukocyte-specific differences associated with obesity, a pilot study examining 5mC in three distinct leukocyte types isolated from peripheral blood of women with normal weight and obesity was conducted.
Methods: CD4 + T cells, CD8 + T cells, and CD16 + neutrophils were reiteratively isolated from blood, and 5mC levels were measured across >450,000 CG sites.
Results: Nineteen CG sites were differentially methylated between women with obesity and with normal weight in CD4 + cells, 16 CG sites in CD8 + cells, and 0 CG sites in CD16 + neutrophils (q < 0.05). There were no common differentially methylated sites between the T-cell types. The amount of visceral adipose tissue was strongly associated with the methylation level of 79 CG sites in CD4 + cells, including 4 CG sites in CLSTN1's promoter, which, this study shows, may regulate its expression.
Conclusions: The methylomes of various leukocytes respond differently to obesity and levels of visceral adipose tissue. Highly significant differentially methylated sites in CD4 + and CD8 + cells in women with obesity that have apparent biological relevance to obesity were identified.
(© 2018 The Obesity Society.)
Databáze: MEDLINE
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