Time-Resolved Imaging of Bacterial Surfaces Using Atomic Force Microscopy.

Autor:	Eskandarian HA; School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.; School of Life Sciences, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., Nievergelt AP; School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., Fantner GE; School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. georg.fantner@epfl.ch.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1814, pp. 385-402.
DOI:	10.1007/978-1-4939-8591-3_23
Abstrakt:	Time-resolved atomic force microscopy (AFM) offers countless new modes by which to study bacterial cell physiology on relevant time scales, from mere milliseconds to hours and days on end. In addition, time-lapse AFM acts as a complementary tool to optical fluorescence microscopy (OFM), for which the combination offers a correlative link between the physical manifestation of bacterial phenotypes and molecular mechanisms obeying those principles. Herein we describe the essential materials and methods necessary for conducting time-resolved AFM and dual AFM/OFM experiments on bacteria.
Databáze:	MEDLINE
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