THE M-RNA, EXPRESSION OF SERCA2 AND NCX1 IN THE PROCESS OF PHARMACOLOGICAL CELL PROTECTION IN EXPERIMENTAL ACUTE PANCREATITIS INDUCED BY TAUROCHOLATE.
Autor: | Vasques ER; Gastroenterology Department LIM 37, University of São Paulo Medical School., Cunha JEM; Gastroenterology Department LIM 37, University of São Paulo Medical School., Kubrusly MS; Gastroenterology Department LIM 37, University of São Paulo Medical School., Coelho AM; Gastroenterology Department LIM 37, University of São Paulo Medical School., Sanpietri SN; Gastroenterology Department LIM 37, University of São Paulo Medical School., Nader HB; Pharmacology Department of Federal University of São Paulo, São Paulo, SP, Brazil., Tersariol ILS; Pharmacology Department of Federal University of São Paulo, São Paulo, SP, Brazil., Lima MA; Pharmacology Department of Federal University of São Paulo, São Paulo, SP, Brazil., Chaib E; Gastroenterology Department LIM 37, University of São Paulo Medical School., D'Albuquerque LAC; Gastroenterology Department LIM 37, University of São Paulo Medical School. |
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Jazyk: | English; Portuguese |
Zdroj: | Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery [Arq Bras Cir Dig] 2018 Jun 21; Vol. 31 (1), pp. e1352. Date of Electronic Publication: 2018 Jun 21. |
DOI: | 10.1590/0102-672020180001e1352 |
Abstrakt: | Background: Intracellular calcium overload is known to be a precipitating factor of pancreatic cell injury in acute pancreatitis (AP). Intracellular calcium homeostasis depends of Plasmatic Membrane Calcium ATPase (PMCA), Sarcoplasmic Endothelial Reticulum Calcium ATPase 2 (SERCA 2) and the Sodium Calcium Exchanger (NCX1). The antioxidant melatonin (Mel) and Trisulfate Disaccharide (TD) that accelerates NCX1 action could reduce the cell damage determined by the AP. Aim: To evaluate m-RNA expressions of SERCA2 and NCX1 in acute pancreatitis induced by sodium taurocholate in Wistar rats pre-treated with melatonin and/or TD. Methods: Wistar rats were divided in groups: 1) without AP; 2) AP without pre-treatment; 3) AP and Melatonin; 4) AP and TD; 5) AP and Melatonin associated to TD. Pancreatic tissue samples were collected for detection of SERCA2 and NCX1 m-R NA levels by polymerase chain reaction (PCR). Results: Increased m-RNA expression of SERCA2 in the melatonin treated group, without increase of m-RNA expression of the NCX1. The TD did not affect levels of SERCA2 and NCX1 m-RNA expressions. The combined melatonin and TD treatment reduced the m-RNA expression of SERCA2. Conclusions: The effect of melatonin is restricted to increased m-RNA expression of SERCA2. Although TD does not affect gene expression, its action in accelerating calcium exchanger function can explain the slightest expression of SERCA2 m-RNA when associated with Melatonin, perhaps by a joint action of drugs with different and but possibly complementary mechanisms. |
Databáze: | MEDLINE |
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