Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis.

Autor:	Voigt A; Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA., Semenova T; Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA., Yamamoto J; Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA., Etienne V; Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA., Nguyen CQ; Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA. nguyenc@ufl.edu.; Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA. nguyenc@ufl.edu.; Center of Orphaned Autoimmune Diseases, University of Florida, Gainesville, FL, USA. nguyenc@ufl.edu.
Jazyk:	angličtina
Zdroj:	Advances in experimental medicine and biology [Adv Exp Med Biol] 2018; Vol. 1068, pp. 89-102.
DOI:	10.1007/978-981-13-0502-3_8
Abstrakt:	Since the discovery of mouse hybridoma technology by Kohler and Milstein in 1975, significant progress has been made in monoclonal antibody production. Advances in B cell immortalization and phage display technologies have generated a myriad of valuable monoclonal antibodies for diagnosis and treatment. Technological breakthroughs in various fields of 'omics have shed crucial insights into cellular heterogeneity of a biological system in which the functional individuality of a single cell must be considered. Based on this important concept, remarkable discoveries in single-cell analysis have made in identifying and isolating functional B cells that produce beneficial therapeutic monoclonal antibodies. In this review, we will discuss three traditional methods of antibody discovery. Recent technological platforms for single-cell antibody discovery will be reviewed. We will discuss the application of the single-cell analysis in finding therapeutic antibodies for human immunodeficiency virus and emerging Zika arbovirus.
Databáze:	MEDLINE
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