Increased extracellular matrix deposition during chondrogenic differentiation of dental pulp stem cells from individuals with neurofibromatosis type 1: an in vitro 2D and 3D study.

Autor: Almeida PN; Graduate Program in Pathology, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.; Neurofibromatosis National Center (Centro Nacional de Neurofibromatose), Rio de Janeiro, Rio de Janeiro, Brazil., Barboza DDN; Oral and Maxillofacial Surgery, Antônio Pedro University Hospital, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Luna EB; Graduate Program in Pathology, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.; Neurofibromatosis National Center (Centro Nacional de Neurofibromatose), Rio de Janeiro, Rio de Janeiro, Brazil., Correia MCM; Dentistry College, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Dias RB; National Institute of Traumatology and Orthopedics (Instituto Nacional de Traumatologia e Ortopedia), Rio de Janeiro, Rio de Janeiro, Brazil., Siquara de Sousa AC; Department of Pathology, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Duarte MEL; National Institute of Traumatology and Orthopedics (Instituto Nacional de Traumatologia e Ortopedia), Rio de Janeiro, Rio de Janeiro, Brazil., Rossi MID; Institute of Biomedical Sciences, and Clementino Fraga Filho University Hospital, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Cunha KS; Graduate Program in Pathology, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. karingcunha@gmail.com.; Neurofibromatosis National Center (Centro Nacional de Neurofibromatose), Rio de Janeiro, Rio de Janeiro, Brazil. karingcunha@gmail.com.
Jazyk: angličtina
Zdroj: Orphanet journal of rare diseases [Orphanet J Rare Dis] 2018 Jun 25; Vol. 13 (1), pp. 98. Date of Electronic Publication: 2018 Jun 25.
DOI: 10.1186/s13023-018-0843-1
Abstrakt: Background: Neurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand cellular and molecular mechanisms underlying NF1 orthopedic problems are of great importance to better understand the pathogenesis and the development of new therapies. Dental pulp stem cells (DPSCs) are being used as an in vitro model for several diseases and appear as a suitable model for NF1. The aim of this study was to evaluate in vitro chondrogenic differentiation of DPSCs from individuals with NF1 using two-dimensional (2D) and three-dimensional (3D) cultures.
Results: To fulfill the criteria of the International Society for Cellular Therapy, DPSCs were characterized by surface antigen expression and by their multipotentiality, being induced to differentiate towards adipogenic, osteogenic, and chondrogenic lineages in 2D cultures. Both DPSCs from individuals with NF1 (NF1 DPSCs) and control cultures were positive for CD90, CD105, CD146 and negative for CD13, CD14, CD45 and CD271, and successfully differentiated after the protocols. Chondrogenic differentiation was evaluated in 2D and in 3D (pellet) cultures, which were further evaluated by optical microscopy and transmission electron microscopy (TEM). 2D cultures showed greater extracellular matrix deposition in NF1 DPSCs comparing with controls during chondrogenic differentiation. In semithin sections, control pellets hadhomogenous-sized intra and extracelullar matrix vesicles, whereas NF1 cultures had matrix vesicles of different sizes. TEM analysis showed higher amount of collagen fibers in NF1 cultures compared with control cultures.
Conclusion: NF1 DPSCs presented increased extracellular matrix deposition during chondrogenic differentiation, which could be related to skeletal changes in individuals with NF1.
Databáze: MEDLINE
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