Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar.

Autor:	San LL; Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Department of Medical Research, Ministry of Health and Sports, Yangon, Myanmar., Aye KS; Department of Medical Research, Ministry of Health and Sports, Yangon, Myanmar., Oo NAT; Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Department of Medical Research, Ministry of Health and Sports, Yangon, Myanmar., Shwe MM; Department of Medical Research, Ministry of Health and Sports, Yangon, Myanmar., Fukushima Y; Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan., Gordon SV; School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland; Hokkaido University, GI-CoRE Global Station for Zoonosis Control, Sapporo, Japan., Suzuki Y; Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Hokkaido University, GI-CoRE Global Station for Zoonosis Control, Sapporo, Japan., Nakajima C; Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Hokkaido University, GI-CoRE Global Station for Zoonosis Control, Sapporo, Japan. Electronic address: cnakajim@czc.hokudai.ac.jp.
Jazyk:	angličtina
Zdroj:	International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2018 Nov; Vol. 76, pp. 109-119. Date of Electronic Publication: 2018 Jun 21.
DOI:	10.1016/j.ijid.2018.06.009
Abstrakt:	Objectives: Myanmar is a World Health Organization high tuberculosis (TB) burden country with a high multidrug-resistant (MDR)-TB burden. Of significance, a high prevalence of the Beijing genotype of Mycobacterium tuberculosis (MTB) among MDR-MTB has been reported previously. A detailed genetic characterization of TB clinical isolates was performed in order to explore whether there is an association between the prevalence of the Beijing MTB genotype and MDR-TB in Myanmar. Methods: A total of 265 MDR-MTB clinical isolates collected in 2010 and 2012 were subjected to spoligotyping, mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) analysis, single nucleotide polymorphism (SNP) typing, and drug resistance-associated gene sequencing, including rpoC to detect potential compensatory evolution. Results: Of the total MDR-MTB isolates, 79.2% (210/265) were of the Beijing genotype, the majority of which were the 'modern' subtype. Beijing genotype isolates were differentiated by 15-locus MIRU-VNTR and a high clustering rate (53.0%) was observed in the modern subtype. These MIRU-VNTR patterns were similar to Beijing genotype clones spreading across Russia and Central Asia. A high prevalence of katG Ser315Thr, and genetic evidence of extensive drug resistance (XDR) and pre-XDR and compensatory mutations in rpoC were observed among clustered isolates. Conclusions: MDR-MTB strains of the Beijing genotype might be spreading in Myanmar and present a major challenge to TB control in this country. (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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