Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide.

Autor: Kinkar L; Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu 51003, Estonia., Laurimäe T; Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu 51003, Estonia., Acosta-Jamett G; Instituto de Medicina Preventiva Veterinaria y Programa de Investigación Aplicada en Fauna Silvestre, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile., Andresiuk V; Laboratorio de Zoonosis Parasitarias, FCEyN, UNMdP, Funes 3350, CP: 7600 Mar del Plata, Buenos Aires, Argentina., Balkaya I; Department of Parasitology, Faculty of Veterinary Medicine, University of Atatürk, Erzurum, Turkey., Casulli A; World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis, European Union Reference Laboratory for Parasites (EURLP), Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy., Gasser RB; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia., González LM; Parasitology Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid 28220, Spain., Haag KL; Departamento de Genética, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Porto Alegre, RS, Brazil., Zait H; Parasitology and Mycology department, Mustapha University Hospital, 16000 Algiers, Algeria., Irshadullah M; Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India., Jabbar A; Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia., Jenkins DJ; School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia., Manfredi MT; Department of Veterinary Medicine, Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy., Mirhendi H; Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., M'rad S; Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), LR 12ES08, Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia., Rostami-Nejad M; Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Oudni-M'rad M; Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), LR 12ES08, Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia., Pierangeli NB; Department of Microbiology and Parasitology, Faculty of Medical Sciences, Comahue National University, Buenos Aires, 1400, 8300 Neuquén, Argentina., Ponce-Gordo F; Department of Parasitology, Faculty of Pharmacy, Complutense University, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain., Rehbein S; Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, 83101 Rohrdorf, Germany., Sharbatkhori M; Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran., Kia EB; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Simsek S; Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey., Soriano SV; Department of Microbiology and Parasitology, Faculty of Medical Sciences, Comahue National University, Buenos Aires, 1400, 8300 Neuquén, Argentina., Sprong H; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and Environment, P.O. Box 1, 3720, BA, Bilthoven, the Netherlands., Šnábel V; Institute of Parasitology, Slovak Academy of Sciences, Košice, Hlinkova 3, 040 01 Košice, Slovakia., Umhang G; ANSES, Nancy Laboratory for Rabies and Wildlife, Wildlife surveillance and eco-epidemiology unit, Malzéville 54220, France., Varcasia A; Laboratory of Parasitology, Veterinary Teaching Hospital, Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy., Saarma U; Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu 51003, Estonia. Electronic address: urmas.saarma@ut.ee.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2018 Oct; Vol. 64, pp. 178-184. Date of Electronic Publication: 2018 Jun 21.
DOI: 10.1016/j.meegid.2018.06.026
Abstrakt: Cystic echinococcosis (CE), a zoonotic disease caused by tapeworms of the species complex Echinococcus granulosus sensu lato, represents a substantial global health and economic burden. Within this complex, E. granulosus sensu stricto (genotypes G1 and G3) is the most frequent causative agent of human CE. Currently, there is no fully reliable method for assigning samples to genotypes G1 and G3, as the commonly used mitochondrial cox1 and nad1 genes are not sufficiently consistent for the identification and differentiation of these genotypes. Thus, a new genetic assay is required for the accurate assignment of G1 and G3. Here we use a large dataset of near-complete mtDNA sequences (n = 303) to reveal the extent of genetic variation of G1 and G3 on a broad geographical scale and to identify reliable informative positions for G1 and G3. Based on extensive sampling and sequencing data, we developed a new method, that is simple and cost-effective, to designate samples to genotypes G1 and G3. We found that the nad5 is the best gene in mtDNA to differentiate between G1 and G3, and developed new primers for the analysis. Our results also highlight problems related to the commonly used cox1 and nad1. To guarantee consistent identification of G1 and G3, we suggest using the sequencing of the nad5 gene region (680 bp). This region contains six informative positions within a relatively short fragment of the mtDNA, allowing the differentiation of G1 and G3 with confidence. Our method offers clear advantages over the previous ones, providing a significantly more consistent means to distinguish G1 and G3 than the commonly used cox1 and nad1.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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