Selection of Structure-Switching DNA Aptamers Binding Soluble Small Molecules and SPR Validation of Enrichment.
| Autor: | Tenaglia E; Institute of Bioengineering, Ecole Polytechnique Fédérale De Lausanne (EPFL), Lausanne, Vaud, Switzerland., Spiga FM; Institute of Bioengineering, Ecole Polytechnique Fédérale De Lausanne (EPFL), Lausanne, Vaud, Switzerland., Guiducci C; Institute of Bioengineering, Ecole Polytechnique Fédérale De Lausanne (EPFL), Lausanne, Vaud, Switzerland. carlotta.guiducci@epfl.ch. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1811, pp. 183-197. |
| DOI: | 10.1007/978-1-4939-8582-1_13 |
| Abstrakt: | Capture-SELEX is an effective molecular strategy enabling the discovery of structure-switching aptamers, which might find useful application in molecular detection or separation. We here provide a protocol to perform capture-SELEX for DNA aptamers binding soluble small molecules, which includes a straightforward functional validation by SPR. The SELEX strategy here described is adaptable to any water-soluble molecular target and might foster the development of DNA aptamers binding therapeutic small molecules, at the great advantage of clinical bioanalytics. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|