Detection of Arbovirus Transmission via Sugar Feeding in a Laboratory Setting.

Autor:	Danforth ME; Davis Arbovirus Research and Training and Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA., Reisen WK; Davis Arbovirus Research and Training and Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA., Barker CM; Davis Arbovirus Research and Training and Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA.
Jazyk:	angličtina
Zdroj:	Journal of medical entomology [J Med Entomol] 2018 Oct 25; Vol. 55 (6), pp. 1575-1579.
DOI:	10.1093/jme/tjy089
Abstrakt:	Most species of mosquitoes consume sugar to survive and during sugar feeding can expectorate virus. Scientists have used this behavior to develop novel methods of mosquito control and arbovirus surveillance. In this study, we use sugar feeding and corresponding viral expectoration to develop an affordable method of monitoring individual mosquitoes for longitudinal data collection. Female Culex tarsalis Coquillett (Diptera: Culicidae) that consumed an infectious bloodmeal of West Nile virus were placed into separate containers and offered a sucrose-soaked cotton wick. Wicks were then collected daily and tested for virus with similar results to those from standard capillary tube method. This yielded a direct longitudinal estimate of the extrinsic incubation period, while using fewer mosquitoes. This approach could be used to further characterize variation in the amount and diversity of expectorated virus over the life span of individual mosquitoes.
Databáze:	MEDLINE
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