Synthesis and application of triclosan methacrylate monomer in resin composites.

Autor: de Paula AB; Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School, University of Campinas, Limeira Avenue, 901, Vila Rezende, Areiao, Piracicaba, São Paulo, 13414-903, Brazil. andbol_63@hotmail.com., Taparelli JR; Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, UNICAMP, Campinas, SP, Brazil., Alonso RCB; Technological and Research Center (NPT), Mogi das Cruzes University (UMC), Mogi das Cruzes, SP, Brazil.; School of Dentistry, Metropolitan University of Santos (UNIMES), Santos, SP, Brazil., Innocentini-Mei LH; Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, UNICAMP, Campinas, SP, Brazil., Puppin-Rontani RM; Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School, University of Campinas, Limeira Avenue, 901, Vila Rezende, Areiao, Piracicaba, São Paulo, 13414-903, Brazil.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2019 Feb; Vol. 23 (2), pp. 965-974. Date of Electronic Publication: 2018 Jun 18.
DOI: 10.1007/s00784-018-2521-z
Abstrakt: Objectives: To evaluate the antibacterial activity, bacterial viability, cytotoxicity, and mechanical/physical properties of a novel methacrylate triclosan-derivative monomer (TM) incorporated in dental resin composite.
Methods: TM was synthesized by esterification and, after characterization by FT-IR, was added to an experimental composite. Samples were divided into two groups according to TM presence, i.e., C1 (control) and C2 (C1 + 14.4% TM). Microbiological properties: Specimens (C1 and C2) were prepared and placed on bacterial suspensions of Streptococcus mutans. Antibacterial activity, MTT, and live/dead bacterial viability were used to test the resin composites. All assays were performed in triplicates. Mechanical properties: Specimens underwent compression (CS) and flexural strength (FS) tests conducted in an Instron universal testing machine at a crosshead speed of 0.5 mm/min. Physical properties: Specimens were assessed for Knoop hardness (KHN) and crosslink density (CD). Fourier transform infrared spectroscopy allowed the degree of conversion (DC) to be evaluated. Data were subjected to appropriate statistical tests according to data distribution and assay (p < 0.05).
Results: Microbiological properties: C2 showed the lowest biofilm accumulation and the highest membrane-compromised bacteria in the biofilm. Mechanical/physical properties: For CS, FS, KHN, and DC, there was no significant difference between groups C1 and C2; however, significant difference was observed for the CD assay.
Conclusions: The triclosan methacrylate reduces bacterial adhesion of S. mutans and decreased the formation of bacterial biofilm without affecting important polymer properties. The triclosan methacrylate incorporated in resin composite could greatly reduce the live bacterial adhesion of S. mutans and decrease the formation of bacterial biofilm without affecting important polymer properties.
Clinical Significance: The resin composites containing triclosan methacrylate could greatly reduce the bacterial adhesion and biofilm formation. That might prevent the secondary caries round the margins of the restorations.
Databáze: MEDLINE
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