Reno-protective effects of TAK-242 on acute kidney injury in a rat model.

Autor: Mohammad BI; College of Pharmacy, University of Al-Qadisiyah, Al-Qadisiyah, Iraq., Raheem AK; College of Medicine, University of Kufa, Al-Najaf, Iraq., Hadi NR; College of Medicine, University of Kufa, Al-Najaf, Iraq., Jamil DA; School of Life Sciences, La Trobe University, Bundoora, VIC, Australia., Al-Aubaidy HA; School of Life Sciences, La Trobe University, Bundoora, VIC, Australia; School of Medicine, University of Tasmania, Hobart, TAS, Australia. Electronic address: h.alaubaidy@latrobe.edu.au.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2018 Sep 03; Vol. 503 (1), pp. 304-308. Date of Electronic Publication: 2018 Jun 13.
DOI: 10.1016/j.bbrc.2018.06.020
Abstrakt: Acute kidney inschemia/reperfusion (I/R) injury is characterized by an abrupt loss of kidney function, resulting in the retention of urea and other nitrogenous waste products and in the dysregulation of extracellular volume and electrolytes. Despite the advances in therapeutic techniques, the mortality and morbidity of patients remain high and have not appreciably improved. This study aims to evaluate the potential protective effect of TAK-242 on renal ischemia/reperfusion injury using an animal model. Thirty-five adult male Sprague-dawely rats (weighing 200-300), were assigned randomly into the following experimental groups (n = 7 in each group), Control (I/R), Sham (negative control), TAK-242 (5 mg/kg body weight), TAK-242 (10 mg/kg body weight) and Vehicle (DMSO). Rats were exposed to a 30 min of ischemia then 3 h of reperfusion. At the end of reperfusion phase, rats were sacrificed then plasma, serum and tissue samples were obtained to measure markers of kidney oxidative stress and inflammation. Plasma levels of neutrophil gelatinase-associated lipocalin (NGAL), and tissue levels of interleukin-18 (IL-18) and malondialdehyde (MDA) were significantly lower in TAK-242 pretreated groups than the vehicle group and the control group (p < 0.05). Furthermore; serum levels of urea and creatinine were significantly lower in the TAK-242 pretreated groups as compared to the control group (p < 0.05). We conclude that administration of TAK-242 can be useful preventive method in attenuating the degree of acute kidney injury during ischemic reperfusion process as shown by a significant reduction of urinary inflammatory markers as well as significant reduction of urea and creatinine levels.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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