Chemical quenching of singlet oxygen by plastoquinols and their oxidation products in Arabidopsis.

Autor: Ferretti U; Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc, 783 71, Czech Republic., Ciura J; Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, 30-387, Poland., Ksas B; Laboratoire d'Écophysiologie Moléculaire des Plantes, CEA, CNRS, UMR 7265 BVME, Aix-Marseille Université, CEA/Cadarache, Saint-Paul-lez-Durance, F-13108, France., Rác M; Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc, 783 71, Czech Republic., Sedlářová M; Department of Botany, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc, 783 71, Czech Republic., Kruk J; Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, 30-387, Poland., Havaux M; Laboratoire d'Écophysiologie Moléculaire des Plantes, CEA, CNRS, UMR 7265 BVME, Aix-Marseille Université, CEA/Cadarache, Saint-Paul-lez-Durance, F-13108, France., Pospíšil P; Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc, 783 71, Czech Republic.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2018 Jun 14. Date of Electronic Publication: 2018 Jun 14.
DOI: 10.1111/tpj.13993
Abstrakt: Prenylquinols (tocochromanols and plastoquinols) serve as efficient physical and chemical quenchers of singlet oxygen ( 1 O 2 ) formed during high light stress in higher plants. Although quenching of 1 O 2 by prenylquinols has been previously studied, direct evidence for chemical quenching of 1 O 2 by plastoquinols and their oxidation products is limited in vivo. In the present study, the role of plastoquinol-9 (PQH 2 -9) in chemical quenching of 1 O 2 was studied in Arabidopsis thaliana lines overexpressing the SOLANESYL DIPHOSPHATE SYNTHASE 1 gene (SPS1oex) involved in PQH 2 -9 and plastochromanol-8 biosynthesis. In this work, direct evidence for chemical quenching of 1 O 2 by plastoquinols and their oxidation products is presented, which is obtained by microscopic techniques in vivo. Chemical quenching of 1 O 2 was associated with consumption of PQH 2 -9 and formation of its various oxidized forms. Oxidation of PQH 2 -9 by 1 O 2 leads to plastoquinone-9 (PQ-9), which is subsequently oxidized to hydroxyplastoquinone-9 [PQ(OH)-9]. We provide here evidence that oxidation of PQ(OH)-9 by 1 O 2 results in the formation of trihydroxyplastoquinone-9 [PQ(OH) 3 -9]. It is concluded here that PQH 2 -9 serves as an efficient 1 O 2 chemical quencher in Arabidopsis, and PQ(OH) 3 -9 can be considered as a natural product of 1 O 2 reaction with PQ(OH)-9. The understanding of the mechanisms underlying 1 O 2 chemical quenching provides information on the role of plastoquinols and their oxidation products in the response of plants to photooxidative stress.
(© 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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