String test: A new tool for tuberculosis diagnosis and drug-resistance detection in children.

Autor:	Imperiale BR; Institute of Experimental Medicine-CONICET-National Academy of Medicine, Buenos Aires, Argentina., Nieves C; Tuberculosis and Mycobacterioses Laboratory, Dr. Cetrangolo Hospital, Buenos Aires, Argentina., Mancino B; Tuberculosis and Mycobacterioses Laboratory, Dr. Cetrangolo Hospital, Buenos Aires, Argentina., Sanjurjo M; Pediatric Unit, Dr. Cetrangolo Hospital, Buenos Aires, Argentina., Tártara S; Infectious Disease Unit, Dr. Cetrangolo Hospital, Buenos Aires, Argentina., Di Giulio ÁB; Mycobacteria Laboratory, Cordero Hospital, Buenos Aires, Argentina., Palomino JC; Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium., Morcillo NS; Tuberculosis and Mycobacterioses Laboratory, Dr. Cetrangolo Hospital, Buenos Aires, Argentina., Martin A; Université catholique de Louvain (UCL), Institute of Experimental and Clinical Research, Laboratory of Medical Microbiology, Brussels, Belgium.
Jazyk:	angličtina
Zdroj:	International journal of mycobacteriology [Int J Mycobacteriol] 2018 Apr-Jun; Vol. 7 (2), pp. 162-166.
DOI:	10.4103/ijmy.ijmy_54_18
Abstrakt:	Background: There is a critical need to improve the diagnostic accuracy of tuberculosis (TB) in children. Several techniques have been developed to improve the quality of sputum samples; however, these procedures are very unpleasant and invasive and require hospitalization and trained personnel. This study aims to explore the potential use of a new and noninvasive tool, "string test," for TB diagnosis in children and in adults not able to render sputum samples and at risk of developing multidrug-resistant TB (MDR-TB). Methods: Children with clinical suspicion of TB attending the pediatric consultation at the Cetrangolo or Cordero Hospitals and adults suspected of MDR-TB and unable to produce sputum attending the Infectious Disease Unit of Cetrangolo Hospital were included in this study. Subjects and Methods: The "string test" is a string that is swallowed by the patients and exposed to gastrointestinal secretions that were late analyzed for TB diagnosis and drug-resistance detection by GenoType MTBDRplus. MedCalc software was used to perform statistical analysis. Results: This technique could be applied on 62.1% of selected children. About 11 (30.6%) children were diagnosed as TB cases, 8 (22.2%) from gastric aspirate and using the "string test." Six out of 19 adults were also diagnosed. Genotype directly on the string specimen detected two MDR-TB in adults and two isoniazid-resistant cases before obtaining the isolate. Conclusion: This test was safe, cheap, and easily implemented without requiring hospitalization. This research could represent a significant step forward to diagnose and rapidly detect drug-resistant TB in children. Competing Interests: There are no conflicts of interest
Databáze:	MEDLINE
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