Phenotypic and functional changes of GM-CSF differentiated human macrophages following exposure to apoptotic neutrophils.

Autor: Chernykh ER; Federal State Budget Scientific Institute «The Research Institute of Fundamental and Clinical Immunology», Yadrintsevskaya Street, 14, Novosibirsk 630099, Russian Federation. Electronic address: ct_lab@mail.ru., Sakhno LV; Federal State Budget Scientific Institute «The Research Institute of Fundamental and Clinical Immunology», Yadrintsevskaya Street, 14, Novosibirsk 630099, Russian Federation. Electronic address: lsahno53@mail.ru., Shevela EY; Federal State Budget Scientific Institute «The Research Institute of Fundamental and Clinical Immunology», Yadrintsevskaya Street, 14, Novosibirsk 630099, Russian Federation. Electronic address: shevelak@mail.ru., Tikhonova MA; Federal State Budget Scientific Institute «The Research Institute of Fundamental and Clinical Immunology», Yadrintsevskaya Street, 14, Novosibirsk 630099, Russian Federation. Electronic address: martix-59@mail.ru., Khonina NA; Federal State Budget Scientific Institute «The Research Institute of Fundamental and Clinical Immunology», Yadrintsevskaya Street, 14, Novosibirsk 630099, Russian Federation. Electronic address: nkhonina@mail.ru., Ostanin AA; Federal State Budget Scientific Institute «The Research Institute of Fundamental and Clinical Immunology», Yadrintsevskaya Street, 14, Novosibirsk 630099, Russian Federation. Electronic address: ostanin62@mail.ru.
Jazyk: angličtina
Zdroj: Cellular immunology [Cell Immunol] 2018 Sep; Vol. 331, pp. 93-99. Date of Electronic Publication: 2018 Jun 07.
DOI: 10.1016/j.cellimm.2018.06.002
Abstrakt: The engulfment of apoptotic cells by monocytes and unprimed macrophages results in M2 polarization. In the current study, we investigated whether apoptotic cells influence the phenotypic and functional characteristics of GM-CSF-differentiated human macrophages (GM-Mφ). Our results demonstrate that GM-Mφ preincubated with apoptotic neutrophils (GM-Mφ Neu ) show significantly increased expression of CD206 and FasL and decreased capacity to stimulate allogeneic T-cell proliferation thus adopting M2 features. The 27-plex analysis demonstrates the down-regulation of 24 cytokines (including IL-10) in GM-Mφ Neu cultures. In contrast, apoptotic neutrophils enhance PGE2 synthesis by GM-Mφ, and blocking PGE2 production with indomethacin restores an allostimulatory activity of GM-MφNeu. These data provide evidence that GM-Mφ following exposure to apoptotic cells acquire features of M2 cells. Given the global suppression of cytokine secretion, GM-Mφ Neu resemble deactivated (M2c) macrophages, and their capacity to inhibit allogeneic T-cell proliferation appears to be mediated by an enhanced synthesis of PGE2 but not IL-10.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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