Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications.

Autor: Baigger A; Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany., Eicke D; Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany., Yuzefovych Y; Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany., Pogozhykh D; Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany., Blasczyk R; Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany., Figueiredo C; Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
Jazyk: angličtina
Zdroj: Journal of cellular and molecular medicine [J Cell Mol Med] 2018 Sep; Vol. 22 (9), pp. 4545-4549. Date of Electronic Publication: 2018 Jun 12.
DOI: 10.1111/jcmm.13698
Abstrakt: Recently, platelet-derived growth factors present in lysates became an extreme interest in the field of regenerative medicine such as in wound healing and as substitutes to foetal bovine serum in xeno-free cell culture systems. However, the generation of such platelet lysates completely depends on the availability of platelet donors. In this study, the possibility to use in vitro-generated megakaryocytes derived from induced pluripotent stem cells (iPSCs) as a cell source for typical platelet growth factors was investigated. Therefore, the presence and levels of those factors were characterized in in vitro-produced megakaryocytes. In comparison with platelets, in vitro-generated megakaryocytes showed a multifold increased content in transcript and protein levels of typical platelet growth factors including platelet-derived growth factors (PDGFs), transforming growth factor (TGF)-1β, vascular endothelial cell factor (VEGF)-A, epidermal growth factor (EGF), insulin-like growth factor (IGF)-1 and tissue factor (TF). Hence, iPSC-derived megakaryocytes may serve as an efficient cell source for a donor-independent generation of growth factor-rich lysates with a broad application potential in innovative cell culture systems and regenerative therapies.
(© 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)
Databáze: MEDLINE
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