Identification of Pasteurella multocida transcribed genes in porcine lungs through RNAseq.

Autor: Chitarra CS; Avenida Fernando Corrêa da Costa, nº 2367 - Bairro Boa Esperança, Cuiabá, Mato Grosso, CEP: 78060-900, Brazil. Electronic address: cristianechitarra@hotmail.com., Oliveira Filho JX; Avenida Paulo Gama, 110 - Bairro Farroupilha, Porto Alegre, Rio Grande do Sul, CEP: 90040-060, Brazil., Morés N; Rodovia BR-153, Km 110, Distrito de Tamanduá Caixa Postal: 321, Concórdia, Santa Catarina, CEP: 89715-899, Brazil., Silva MIVD; Avenida Fernando Corrêa da Costa, nº 2367 - Bairro Boa Esperança, Cuiabá, Mato Grosso, CEP: 78060-900, Brazil., Cândido SL; Avenida Fernando Corrêa da Costa, nº 2367 - Bairro Boa Esperança, Cuiabá, Mato Grosso, CEP: 78060-900, Brazil., Cezarino PG; Avenida Fernando Corrêa da Costa, nº 2367 - Bairro Boa Esperança, Cuiabá, Mato Grosso, CEP: 78060-900, Brazil., Nakazato L; Avenida Fernando Corrêa da Costa, nº 2367 - Bairro Boa Esperança, Cuiabá, Mato Grosso, CEP: 78060-900, Brazil., Dutra V; Avenida Fernando Corrêa da Costa, nº 2367 - Bairro Boa Esperança, Cuiabá, Mato Grosso, CEP: 78060-900, Brazil.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2018 Sep; Vol. 122, pp. 180-183. Date of Electronic Publication: 2018 Jun 08.
DOI: 10.1016/j.micpath.2018.06.014
Abstrakt: Pasteurella multocida is one of the most important pathogen that causes pneumonia in swine. Although several virulence factors are known, the pathogenesis of this bacterium is not well-studied. Therefore, to study the pathogenesis of P. multocida infection in porcine lung, next-generation RNA sequencing was used to compare the transcriptomes of P. multocida grown in vivo and in vitro, respectively. After P. multocida infection a total of 704 genes were expressed in vitro, 1422 genes were expressed in vivo, and 237 genes were differentially expressed based on statistical analyses, padj of ≤0.1. Genes encoding ribosomal proteins or other products that function in the regulation of transcription and translation were downregulated, whereas genes whose products affected cellular processes (protein transport and RNA degradation) and metabolic pathways, such as those of amino acid metabolism and nucleotide metabolism, were upregulated in vitro compared with in vivo. This study shows that differentially expressed genes in P. multocida regulate pathways that operate during stress, iron capture, heat shock, and nitrogen regulation. However, extensive investigation of the pathogenic mechanism of P. multocida is still required.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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