Real-time assessment of bacteriophage T3-derived antimicrobial activity against planktonic and biofilm-embedded Escherichia coli by isothermal microcalorimetry.

Autor: Tkhilaishvili T; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies Charité, University Medicine Berlin, Augustenburger Platz 1 (Südstraße 2) 13353 Berlin, Germany., Di Luca M; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies Charité, University Medicine Berlin, Augustenburger Platz 1 (Südstraße 2) 13353 Berlin, Germany; NEST, Istituto Nanoscienze, Consiglio Nazionale delle Ricerche, Piazza San Silvestro 12, 56127, Pisa, Italy. Electronic address: diluca.mariagrazia@gmail.com., Abbandonato G; NEST, Istituto Nanoscienze, Consiglio Nazionale delle Ricerche, Piazza San Silvestro 12, 56127, Pisa, Italy., Maiolo EM; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies Charité, University Medicine Berlin, Augustenburger Platz 1 (Südstraße 2) 13353 Berlin, Germany., Klatt AB; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies Charité, University Medicine Berlin, Augustenburger Platz 1 (Südstraße 2) 13353 Berlin, Germany., Reuter M; Institute of Virology, Helmut-Ruska-Haus, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany., Möncke-Buchner E; Institute of Virology, Helmut-Ruska-Haus, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany., Trampuz A; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies Charité, University Medicine Berlin, Augustenburger Platz 1 (Südstraße 2) 13353 Berlin, Germany.
Jazyk: angličtina
Zdroj: Research in microbiology [Res Microbiol] 2018 Nov; Vol. 169 (9), pp. 515-521. Date of Electronic Publication: 2018 Jun 07.
DOI: 10.1016/j.resmic.2018.05.010
Abstrakt: Bacterial biofilms, highly resistant to the conventional antimicrobial therapy, remain an unresolved challenge pressing the medical community to investigate new and alternative strategies to fight chronic implant-associated infections. Recently, strictly lytic bacteriophages have been revalued as powerful agents to kill antibiotic-resistant bacteria even in biofilm. Here, the interaction of T3 bacteriophage and planktonic and biofilm Escherichia coli TG1, respectively, was evaluated using isothermal microcalorimetry. Microcalorimetry is a non-invasive and highly sensitive technique measuring growth-related heat production of microorganisms in real-time. Planktonic and biofilm E. coli TG1 were exposed to different titers of T3 bacteriophage, ranging from 10 2 to 10 7  PFU/ml. The incubation of T3 with E. coli TG1 showed a strong inhibition of heat production both in planktonic and biofilm already at lower bacteriophage titers (10 3  PFU/ml). This method could be used to screen and evaluate the antimicrobial potential of different bacteriophages, alone and in combination with antibiotics in order to improve the treatment success of biofilm-associated infections.
(Copyright © 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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