Functional and proteomic comparison of different techniques to produce equine anti-tetanus immunoglobulin F(ab')2 fragments.

Autor: Zhang XJ; Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, Sichuan province, PR China. Electronic address: hotbird007@163.com., Li HL; College of Environment and Ecology, Chengdu University of Technology, Chengdu, Sichuan province, PR China; West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, Sichuan province, PR China., Deng DY; Jiangxi Institute of Biological Products, Ji'an, Jiangxi province, PR China., Ji C; Jiangxi Institute of Biological Products, Ji'an, Jiangxi province, PR China., Yao XD; Jiangxi Institute of Biological Products, Ji'an, Jiangxi province, PR China., Liu JX; Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, Sichuan province, PR China.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2018 Aug 15; Vol. 1092, pp. 29-39. Date of Electronic Publication: 2018 May 29.
DOI: 10.1016/j.jchromb.2018.05.041
Abstrakt: Tetanus is still a major cause of human deaths in several developing countries. In particular, the neonatal form remains a significant public health problem. According to the World Health Organization, administration of tetanus toxoid is recommended for neonatal tetanus patients. Furthermore, tetanus antitoxin or anti-tetanus immunoglobulin (Ig) are used for mild case or intensive care. This paper discusses a novel purification technique for improving equine anti-tetanus Ig production. First, equine plasma dealt with two steps salting out with ammonium sulfate; second, ultrafiltration concentration liquid purified by one successive protein G based affinity chromatography steps; finally, the purified F(ab')2 fragments was characterized using biochemical and proteomic methods and shown to be pure and homogeneous. Compared with the original technique product, specific activity increased by 80% (about 90,000 IU/g) and recovery of F(ab')2 is approximately equal 75%. Furthermore, Proteomic profiling of total technique process is demonstrated by nano-HPLC-MS and bioinformatics analysis. New technique to produce equine anti-tetanus immunoglobulin F(ab')2 fragments from crude plasma in high quality and yield. And it also could be used for industrial amplification.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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