Mutagenic effects of spent potliner and derivatives on Allium cepa L. and Lactuca sativa L.: A molecular approach.

Autor: Andrade-Vieira LF; Department of Biology, Federal University of Lavras (Universidade Federal de Lavras), Lavras, MG, 37.200-000, Brazil. Electronic address: lfandrade.vieira@gmail.com., Bernardes PM; Department of Agronomy, Center of Exact Sciences and Engineering, Federal University of Espírito Santo (Universidade Federal do Espírito Santo), Alegre, ES, 29.500-000, Brazil., Ferreira MFDS; Department of Agronomy, Center of Exact Sciences and Engineering, Federal University of Espírito Santo (Universidade Federal do Espírito Santo), Alegre, ES, 29.500-000, Brazil.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2018 Oct; Vol. 208, pp. 257-262. Date of Electronic Publication: 2018 May 30.
DOI: 10.1016/j.chemosphere.2018.05.186
Abstrakt: Spent potliner (SPL) is a solid residue generated by the aluminum industry. Its composition is variable and complex, containing fluoride and cyanide salts as well as aluminum, which contributes to its toxicity. SPL is sometimes released directly into the soil, where it is prone to leaching and has the potential to cause alterations and damage to DNA. Considering that polymorphism analysis of simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR) DNA markers is an interesting tool to determine the mutagenicity of an environmental pollutant, the present study adopted this approach to verify the mutagenic potential of SPL and its main toxic components (aluminum, fluoride, and cyanide) on root tip cells of Lactuca sativa and Allium cepa. Alterations in ISSR and SSR regions were identified by DNA fingerprinting (gain and loss of bands and changes in band intensity). The estimated dissimilarities indicated differences between treatments and the negative control. Furthermore, the relationship between the amplification profile of the markers and alterations in cell mitosis was discussed.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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