| Autor: |
Lim X; Tissue Engineering Group, Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, ORCRB, Roosevelt Drive, Headington, Oxford, OX3 7DQ, UK., Potter M; Department of Plastic Surgery, Oxford University Hospitals NHS Foundation Trust, West Wing, John Radcliffe Hospital, Headington, Oxford, OX9 9DU, UK., Cui Z; Tissue Engineering Group, Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, ORCRB, Roosevelt Drive, Headington, Oxford, OX3 7DQ, UK., Dye JF; Tissue Engineering Group, Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, ORCRB, Roosevelt Drive, Headington, Oxford, OX3 7DQ, UK. Julian.dye@eng.ox.ac.uk. |
| Abstrakt: |
There are significant challenges for using emulsion templating as a method of manufacturing macro-porous protein scaffolds. Issues include protein denaturation by adsorption at hydrophobic interfaces, emulsion instability, oil droplet and surfactant removal after protein gelation, and compatible cross-linking methods. We investigated an oil-in-water macro-emulsion stabilised with a surfactant blend, as a template for manufacturing protein-based nano-structured bio-intelligent scaffolds (EmDerm) with tuneable micro-scale porosity for tissue regeneration. Prototype EmDerm scaffolds were made using either collagen, through thermal gelation, fibrin, through enzymatic coagulation or collagen-fibrin composite. Pore size was controlled via surfactant-to-oil phase ratio. Scaffolds were crosslink-stabilised with EDC/NHS for varying durations. Scaffold micro-architecture and porosity were characterised with SEM, and mechanical properties by tensiometry. Hydrolytic and proteolytic degradation profiles were quantified by mass decrease over time. Human dermal fibroblasts, endothelial cells and bone marrow derived mesenchymal stem cells were used to investigate cytotoxicity and cell proliferation within each scaffold. EmDerm scaffolds showed nano-scale based hierarchical structures, with mean pore diameters ranging from 40-100 microns. The Young's modulus range was 1.1-2.9 MPa, and ultimate tensile strength was 4-16 MPa. Degradation rate was related to cross-linking duration. Each EmDerm scaffold supported excellent cell ingress and proliferation compared to the reference materials Integra™ and Matriderm™. Emulsion templating is a novel rapid method of fabricating nano-structured fibrous protein scaffolds with micro-scale pore dimensions. These scaffolds hold promising clinical potential for regeneration of the dermis and other soft tissues, e.g., for burns or chronic wound therapies. |
Full text from SpringerLink