| Autor: |
Oliveira RM; Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. ruth.oliveira@ifrn.edu.br.; Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte, Caicó, Rio Grande do Norte 59.300-000, Brazil. ruth.oliveira@ifrn.edu.br., Câmara RBG; Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte, Caicó, Rio Grande do Norte 59.300-000, Brazil. rafael_bgc@yahoo.com.br.; Escola Multicampi de Ciências Médicas, Universidade Federal do Rio Grande do Norte, Caicó, Rio Grande do Norte 59.300-000, Brazil. rafael_bgc@yahoo.com.br., Monte JFS; Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. jessykamonte@gmail.com., Viana RLS; Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. rony_lucas@hotmail.com., Melo KRT; Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. melo.krt@gmail.com., Queiroz MF; Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. moacirfqn@gmail.com., Filgueira LGA; Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. lucianagalves@hotmail.com., Oyama LM; Departamento de Fisiologia, Universidade Federal de São Paulo-Escola Paulista de Medicina, São Paulo 04023-060, Brazil. lmoyama@gmail.com., Rocha HAO; Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. hugo@cb.ufrn.br. |
| Abstrakt: |
Fucus vesiculosus is a brown seaweed used in the treatment of obesity. This seaweed synthesizes various bioactive molecules, one of them being a sulfated polysaccharide known as fucoidan (FF). This polymer can easily be found commercially, and has antiadipogenic and lipolytic activity. Using differential precipitation with acetone, we obtained four fucoidan-rich fractions (F0.5/F0.9/F1.1/F2.0) from FF. These fractions contain different proportions of fucose:glucuronic acid:galactose:xylose:sulfate, and also showed different electrophoretic mobility and antioxidant activity. Using 3T3-L1 adipocytes, we found that all samples had lipolytic action, especially F2.0, which tripled the amount of glycerol in the cellular medium. Moreover, we observed that FF, F1.0, and F2.0 have antiadipogenic activity, as they inhibited the oil red staining by cells at 40%, 40%, and 50%, respectively. In addition, they decreased the expression of key proteins of adipogenic differentiation (C/EBPα, C/EBPβ, and PPARγ). However, F0.5 and F0.9 stimulated the oil red staining at 80% and increased the expression of these proteins. Therefore, these fucoidan fractions have an adipogenic effect. Overall, the data show that F2.0 has great potential to be used as an agent against obesity as it displays better antioxidant, lipolytic and antiadipogenic activities than the other fucoidan fractions that we tested. |
