Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana.

Autor:	Dealtry S; Pontifícia Universidade Católica do Rio de Janeiro, Departamento de Engenharia Civil, Rio de Janeiro, RJ, Brazil; Universidade Federal do Rio de Janeiro, Programa de Pós-Graduação em Biotecnologia Vegetal, Rio de Janeiro, RJ, Brazil. Electronic address: simonedgs@yahoo.com.br., Ghizelini AM; Universidade Federal do Rio de Janeiro, Programa de Pós-Graduação em Biotecnologia Vegetal, Rio de Janeiro, RJ, Brazil; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Rio de Janeiro, RJ, Brazil., Mendonça-Hagler LCS; Universidade Federal do Rio de Janeiro, Programa de Pós-Graduação em Biotecnologia Vegetal, Rio de Janeiro, RJ, Brazil; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Rio de Janeiro, RJ, Brazil., Chaloub RM; Universidade Federal do Rio de Janeiro, Instituto de Química, Departamento de Bioquímica Rio de Janeiro, RJ, Brazil., Reinert F; Universidade Federal do Rio de Janeiro, Programa de Pós-Graduação em Biotecnologia Vegetal, Rio de Janeiro, RJ, Brazil; Universidade Federal do Rio de Janeiro, Instituto de Biologia, Laboratório de Fisologia de Planta, Rio de Janeiro, RJ, Brazil., Campos TMP; Pontifícia Universidade Católica do Rio de Janeiro, Departamento de Engenharia Civil, Rio de Janeiro, RJ, Brazil., Gomes NCM; Universidade de Aveiro, Departamento de Biologia, Centro de Estudos do Ambiente e do Mar (CESAM), Aveiro, Portugal., Smalla K; Julius Kühn-Institut (JKI) Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Braunschweig, Germany.
Jazyk:	angličtina
Zdroj:	Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2018 Oct - Dec; Vol. 49 (4), pp. 757-769. Date of Electronic Publication: 2018 Jun 14.
DOI:	10.1016/j.bjm.2018.02.012
Abstrakt:	Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while the bacterial community was rather stable, pronounced shifts were observed in the abundance of bacteria carrying plasmids. A PCR-Southern blot hybridization analysis indicated an increase in the abundance of IncP-9 catabolic plasmids. Denaturing gradient gel electrophoresis of naphthalene dioxygenase (ndo) genes amplified from cDNA (RNA) indicated the dominance of a specific ndo gene in the inoculated petroleum amendment treatment. The petroleum hydrocarbon degrading consortium characterization indicated the prevalence of bacteria assigned to Pseudomonas spp., Comamonas spp. and Ochrobactrum spp. IncP-9 plasmids were detected for the first time in Comamonas sp. and Ochrobactrum spp., which is a novelty of this study. (Copyright © 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.)
Databáze:	MEDLINE
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