NtTPN1: a RPP8-like R gene required for Potato virus Y-induced veinal necrosis in tobacco.

Autor: Michel V; UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, 71 Av. E. Bourlaux, CS 20032, 33882, Villenave d'Ornon Cedex, France., Julio E; Imperial Tobacco, La Tour, 24100, Bergerac, France., Candresse T; UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, 71 Av. E. Bourlaux, CS 20032, 33882, Villenave d'Ornon Cedex, France., Cotucheau J; Imperial Tobacco, La Tour, 24100, Bergerac, France., Decorps C; Imperial Tobacco, La Tour, 24100, Bergerac, France., Volpatti R; Imperial Tobacco, La Tour, 24100, Bergerac, France., Moury B; Pathologie Végétale, INRA, 84140, Montfavet, France., Glais L; FN3PT/RD3PT, 75008, Paris, France.; IGEPP, Agrocampus Ouest, INRA, Université de Rennes 1, 35650, Le Rheu, France., Dorlhac de Borne F; Imperial Tobacco, La Tour, 24100, Bergerac, France., Decroocq V; UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, 71 Av. E. Bourlaux, CS 20032, 33882, Villenave d'Ornon Cedex, France., German-Retana S; UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, 71 Av. E. Bourlaux, CS 20032, 33882, Villenave d'Ornon Cedex, France.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2018 Jun 04. Date of Electronic Publication: 2018 Jun 04.
DOI: 10.1111/tpj.13980
Abstrakt: Potato virus Y (PVY) is one of the most damaging viruses of tobacco. In particular, aggressive necrotic strains (PVY N ) lead to considerable losses in yield. The main source of resistance against PVY is linked to the va locus. However, va-overcoming PVY isolates inducing necrotic symptoms were observed in several countries. In this context, it is important to find va-independent protection strategies. In a previous study, the phenotyping of 162 tobacco varieties revealed 10 accessions that do not carry the va allele and do not exhibit typical PVY N -induced veinal necrosis. Despite the absence of necrotic symptoms, normal viral accumulation in these plants suggests a va-independent mechanism of tolerance to PVY N -induced systemic veinal necrosis. Fine mapping of the genetic determinant(s) was performed in a segregating F2 population. The tolerance trait is inherited as a single recessive gene, and allelism tests demonstrated that eight of the 10 tolerant varieties carry the same determinant. Anchoring the linkage map to the tobacco genome physical map allowed the identification of a RPP8-like R gene, called NtTPN1 (for Nicotiana tabacum Tolerance to PVY-induced Necrosis1), with the same single-nucleotide polymorphism in the eight tolerant accessions. Functional assays using homozygous NtTPN1 EMS mutants confirmed the role of NtTPN1 in the tolerance phenotype. PVY N -induced systemic veinal necrosis in tobacco likely represents an inefficient defense response with hypersensitive response-like characteristics. The identification of NtTPN1 opens breeding options to minimize the impact of emerging and so far uncontrolled va-breaking necrotic PVY isolates.
(© 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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