The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria.

Autor: Seni J; Department of Microbiology and Immunology, Catholic University of Health and Allied Sciences, Mwanza, Tanzania; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary,Calgary, Alberta, Canada., Peirano G; Department of Pathology & Laboratory Medicine, Cumming School of Medicine, University of Calgary,Calgary, Alberta, Canada; Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada., Okon KO; Department of Medical Microbiology, Federal Medical Centre, Makurdi., Jibrin YB; Department of Internal Medicine, Abubakar Tafawa Balewa University teaching hospital Bauchi., Mohammed A; Department of Internal Medicine, Abubakar Tafawa Balewa University teaching hospital Bauchi., Mshana SE; Department of Microbiology and Immunology, Catholic University of Health and Allied Sciences, Mwanza, Tanzania., DeVinney R; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary,Calgary, Alberta, Canada., Pitout JDD; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary,Calgary, Alberta, Canada; Department of Pathology & Laboratory Medicine, Cumming School of Medicine, University of Calgary,Calgary, Alberta, Canada; Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada; Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa. Electronic address: johann.pitout@cls.ab.ca.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2018 Sep; Vol. 92 (1), pp. 46-49. Date of Electronic Publication: 2018 Apr 14.
DOI: 10.1016/j.diagmicrobio.2018.04.001
Abstrakt: Limited information is available regarding the population structure of extra-intestinal pathogenic Escherichia coli (ExPEC) in Africa. Antimicrobial resistance profiles, sequence types (STs) and fimH types were determined on 60 clinical ExPEC from Nigeria using a 7-single nucleotide polymorphism quantitative PCR and sequencing of certain genes. Different ST131 clades were identified with a multiplex PCR. The isolates were mostly obtained from urines (58.3%). Not-susceptibility rates were as follows: trimethoprim-sulfamethoxazole (98%), cefotaxime (68%), gentamicin (55%), ciprofloxacin (62%) and piperacillin-tazobactam (2%). Dominant STs were associated with CTX-M-15 and included ST131-fimH30 (23%), ST457-fimH145 (20%), ST405-fimH27 (13%) and ST95-fimH41 (10%). We found the 7-SNP qPCR to be simple and cost-effective that can be utilized to tract different ExPEC clones on a global scale. This study provided insight into the population structure of ExPEC from Nigeria showing high prevalence of the rarely reported ST457 and the presence of multidrug resistant ST95.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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