Hepatoprotective effect of lawsone on rifampicin-isoniazid induced hepatotoxicity in in vitro and in vivo models.

Autor: Darvin SS; Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, 600034, India. Electronic address: sylvestrdarvin14deri03@loyolacollege.edu., Esakkimuthu S; Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, 600034, India. Electronic address: esakkimuthu14deri05@loyolacollege.edu., Toppo E; Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, 600034, India. Electronic address: erentoppo@loyolacollege.edu., Balakrishna K; Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, 600034, India. Electronic address: drkbalki@yahoo.co.in., Paulraj MG; Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, 600034, India. Electronic address: gabrielpaulraj@loyolacollege.edu., Pandikumar P; Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, 600034, India. Electronic address: pandikumar@loyolacollege.edu., Ignacimuthu S; Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, 600034, India; International Scientific Partnership Programme, King Saud University, Riyadh, 11451, Saudi Arabia. Electronic address: eri@loyolacollege.edu., Al-Dhabi NA; Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O Box 2455, Riyadh 11451, Saudi Arabia. Electronic address: naldhabi@ksu.edu.sa.
Jazyk: angličtina
Zdroj: Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2018 Jul; Vol. 61, pp. 87-94. Date of Electronic Publication: 2018 May 15.
DOI: 10.1016/j.etap.2018.05.006
Abstrakt: The Drug-induced liver injury is one of the common unfavourable impacts, which seriously affects any drug therapy. This study documented the hepatoprotective efficacy of lawsone, the major bioactive naphthoquinone present in Lawsonia inermis L. (Lythraceae) using in vitro and in vivo models. Lawsone was isolated from the leaves of L. inermis and its structure was confirmed using spectroscopic data. In-vitro antioxidant effect of lawsone was evaluated using ABTS assay. Hepatoprotective effect of lawsone was determined with RIF-INH treated HepG2 cells and Wistar rats. Administration of RIF-INH reduced the viability of the HepG2 cells and the treatment with lawsone significantly restored the viability of the cells even at lower concentration (7.5 μM). The other parameters such as the leakage of transaminases and MDA levels were also significantly reduced by the treatment with lawsone. Oral administration of lawsone to the animals did not show any toxicity up to 2 g/kg b.w. concentration. Treatment with lawsone to the RIF-INH administered animals significantly lowered the serum transaminases levels. The ratio of albumin to globulin was improved and the level of bilirubin was lowered. This study indicated the hepatoprotective effect of lawsone; detailed investigations will give deeper understanding of the application of lawsone for hepatoprotection.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: