Synthesis and secretion of volatile short-chain fatty acids in Triatoma infestans infected with Beauveria bassiana.

Autor: Lobo LS; Instituto de Investigaciones Bioquímicas de La Plata (CCT La Plata CONICET-UNLP), Facultad de Ciencias Médicas, La Plata, Buenos Aires, Argentina.; Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Goiás, Brazil., Girotti JR; Instituto de Investigaciones Bioquímicas de La Plata (CCT La Plata CONICET-UNLP), Facultad de Ciencias Médicas, La Plata, Buenos Aires, Argentina., Mijailovsky SJ; Instituto de Investigaciones Bioquímicas de La Plata (CCT La Plata CONICET-UNLP), Facultad de Ciencias Médicas, La Plata, Buenos Aires, Argentina., Fernandes ÉKK; Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Goiás, Brazil., Luz C; Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Goiás, Brazil., Pedrini N; Instituto de Investigaciones Bioquímicas de La Plata (CCT La Plata CONICET-UNLP), Facultad de Ciencias Médicas, La Plata, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Medical and veterinary entomology [Med Vet Entomol] 2018 Sep; Vol. 32 (3), pp. 358-364. Date of Electronic Publication: 2018 Jun 02.
DOI: 10.1111/mve.12306
Abstrakt: Physically disturbed Triatoma infestans (Hemiptera: Reduviidae) adults, as well as adults of other Chagas' disease vectors, secrete a mix of volatile organic compounds (VOCs) with alarm and possible sexual and defence functions. The aim of the present research was to test whether infection with the entomopathogenic fungus Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae) has an effect on VOC secretion in disturbed T. infestans and on the expression of two genes (Ti-brnq and Ti-bckdc) potentially involved in VOC biosynthesis. The volatiles released by insects at different time periods after fungal treatment were identified and their relative amounts measured. Isobutyric acid was the most abundant volatile found in both healthy and fungus-infected insects and underwent no significant relative changes through the infection process. The secretion of propionic acid, however, was significantly higher at 1-4 days post-infection (d.p. i.) compared with that in controls. A slight induction of both Ti-brnq and Ti-bckdc genes was found by real-time polymerase chain reaction at 4 d.p. i., with expression values reaching up to three-fold those in controls. The early stages of fungal infection seem to affect the composition of the alarm pheromone by changing the expression pattern of both genes analysed. These results help to elucidate the impact of fungal infections on the chemical ecology of triatomine bugs.
(© 2018 The Royal Entomological Society.)
Databáze: MEDLINE
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