| Autor: |
Kaushik R; Department of Chemistry , National Institute of Technology (NIT) Kurukshetra , Kurukshetra - 136119 , Haryana , India., Sakla R; Department of Chemistry , National Institute of Technology (NIT) Kurukshetra , Kurukshetra - 136119 , Haryana , India., Ghosh A; Department of Chemistry , National Institute of Technology (NIT) Kurukshetra , Kurukshetra - 136119 , Haryana , India., Selvan GT; Department of Science & Humanities , Karunya Institute of Technology & Sciences , Coimbatore 641114 , Tamil Nadu , India., Selvakumar PM; Department of Science & Humanities , Karunya Institute of Technology & Sciences , Coimbatore 641114 , Tamil Nadu , India., Jose DA; Department of Chemistry , National Institute of Technology (NIT) Kurukshetra , Kurukshetra - 136119 , Haryana , India. |
| Abstrakt: |
A new approach for the detection of hydrogen sulfide (H 2 S) was constructed within vesicles comprising phospholipids and amphiphilic copper complex as receptor. 1,2-Distearoyl- sn-glycero-3-phosphocholine (DSPC) vesicles with embedded metal complex receptor (1.Cu) sites have been prepared. The vesicles selectively respond to H 2 S in a buffered solution and show colorimetric as well as spectral transformation. Other analytes such as reactive sulfur species, reactive nitrogen species, biological phosphates, and other anions failed to induce changes. The H 2 S detection is established through a metal indicator displacement (MIDA) process, where Eosin-Y (EY) was employed as an indicator. Fluorescence, UV-vis spectroscopy, and the naked eye as the signal readout studies confirm the high selectivity, sensitivity, and lower detection limit of the vesicular receptor. The application of vesicular receptors for real sample analysis was also confirmed by fluorescence live cell imaging. |
