| Autor: |
Cannella D; Department of Geoscience and Natural Resources, University of Copenhagen, Frederiksberg C, Denmark. david.cannella@ulb.ac.be.; Interfaculty School of Bioengineers, Universite Libre de Bruxelles ULB, Bruxelles, Belgium. david.cannella@ulb.ac.be. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1796, pp. 247-253. |
| DOI: |
10.1007/978-1-4939-7877-9_17 |
| Abstrakt: |
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes that oxidize the most recalcitrant polysaccharides and require extracellular electron donors. The role of electron donation to redox enzymes is pivotal since a nonefficient electron transfer might result in partial activity or reduced kinetics. In this protocol we show the effect of using excited photosynthetic pigments combined with reducing agents as efficient electron donors for monooxygenases. The light-induced electron transfer can enhance the oxidation ability of LPMOs up to ten times. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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