Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger.

Autor: Reilly MC; Joint BioEnergy Institute, Emeryville, CA, USA.; University of California, San Francisco, CA, USA., Amaike Campen S; Joint BioEnergy Institute, Emeryville, CA, USA.; J. Craig Venter Institute, San Diego, CA, USA., Simmons BA; Joint BioEnergy Institute, Emeryville, CA, USA.; Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Baker SE; Joint BioEnergy Institute, Emeryville, CA, USA.; Pacific Northwest National Laboratory, Richland, WA, USA., Gladden JM; Joint BioEnergy Institute, Emeryville, CA, USA.; Sandia National Laboratory, Livermore, CA, USA., Magnuson JK; Joint BioEnergy Institute, Emeryville, CA, USA. jon.magnuson@pnnl.gov.; Pacific Northwest National Laboratory, Richland, WA, USA. jon.magnuson@pnnl.gov.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1796, pp. 123-133.
DOI: 10.1007/978-1-4939-7877-9_10
Abstrakt: Cellulases and other enzymes are needed for saccharification of plant biomass in the biorefinery industry. Expression, characterization, and eventual large-scale production of known and novel cellulases requires the ability to express and secrete heterologous enzymes in relevant protein production platforms like Aspergillus niger. A method for cloning and expression of genes for these desirable enzymes in A. niger is presented in this Chapter.
Databáze: MEDLINE