Dissection and Preparation of Human Primary Fetal Ganglionic Eminence Tissue for Research and Clinical Applications.

Autor:	Roberton VH; Brain Repair Group, School of Biosciences, Cardiff University, Cardiff, UK., Rosser AE; Brain Repair Group, Cardiff University, Cardiff, UK., McGorrian AM; Brain Repair Group, School of Biosciences, Cardiff University, Cardiff, UK., Precious SV; Brain Repair Group, Cardiff University, Cardiff, UK. precioussv@cardiff.ac.uk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1780, pp. 573-583.
DOI:	10.1007/978-1-4939-7825-0_26
Abstrakt:	Here, we describe detailed dissection and enzymatic dissociation protocols for the ganglionic eminences from the developing human brain to generate viable quasi-single cell suspensions for subsequent use in transplantation or cell culture. These reliable and reproducible protocols can provide tissue for use in the study of the developing human brain, as well as for the preparation of donor cells for transplantation in Huntington's disease (HD). For use in the clinic as a therapy for HD, the translation of these protocols from the research laboratory to the GMP suite is described, including modification to reagents used and appropriate monitoring and tissue release criteria.
Databáze:	MEDLINE
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