Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules.

Autor:	Wang Y; School of Biological Sciences, University of Wollongong, Wollongong, NSW, Australia., Letham DS; Research School of Biology, Australian National University, Canberra, ACT, Australia., John PCL; Research School of Biology, Australian National University, Canberra, ACT, Australia., Zhang R; School of Biological Sciences, University of Wollongong, Wollongong, NSW, Australia. rzhang@uow.edu.au.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1794, pp. 225-234.
DOI:	10.1007/978-1-4939-7871-7_14
Abstrakt:	Protein-small molecule interaction studies provide useful insights into biological processes taking place within the living cell. A special yeast hybrid system, the yeast three-hybrid method, has been developed and used to explore proteins that bind to small molecules, by which means it may be possible to unravel biological processes and dissect function of biological systems. Here we present a protocol employing this method for identifying such binding proteins.
Databáze:	MEDLINE
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