Micromolecular methods for diagnosis and therapeutic strategy: a case study.

Autor:	Elbouchtaoui M; Université Paris Diderot, Inserm, UMR_S1165, Paris, France.; Pathology Department, Hôpital St Louis, APHP, Paris, France., Tengher I; Pathology Department, Hôpital Jean Verdier, APHP, Bondy, France., Miquel C; Université Paris Diderot, Inserm, UMR_S1165, Paris, France.; Pathology Department, Hôpital St Louis, APHP, Paris, France., Brugière C; Université Paris Diderot, Inserm, UMR_S1165, Paris, France., Benbara A; Obstetrics and Gynecology Department, Hôpital Jean Verdier, APHP, Bondy, France., Zelek L; Université Paris 13, Villetaneuse, France.; Oncology Department, Hôpital Avicenne, APHP, Bobigny, France., Ziol M; Pathology Department, Hôpital Jean Verdier, APHP, Bondy, France.; Université Paris 13, Villetaneuse, France.; Inserm UMR_S1162, Paris, France., Bouhidel F; Université Paris Diderot, Inserm, UMR_S1165, Paris, France.; Pathology Department, Hôpital St Louis, APHP, Paris, France., Janin A; Université Paris Diderot, Inserm, UMR_S1165, Paris, France.; Pathology Department, Hôpital St Louis, APHP, Paris, France., Bousquet G; Université Paris Diderot, Inserm, UMR_S1165, Paris, France.; Université Paris 13, Villetaneuse, France.; Oncology Department, Hôpital Avicenne, APHP, Bobigny, France., Leboeuf C; Université Paris Diderot, Inserm, UMR_S1165, Paris, France.
Jazyk:	angličtina
Zdroj:	Oncotarget [Oncotarget] 2018 Apr 27; Vol. 9 (32), pp. 22862-22869. Date of Electronic Publication: 2018 Apr 27 (Print Publication: 2018).
DOI:	10.18632/oncotarget.25161
Abstrakt:	An intraductal carcinoma, 55 mm across, was diagnosed on a total mastectomy in a 45-year-old woman. The 2 micro-invasive areas found were too small for reliable immunostainings for estrogen, progesterone, and HER2 receptors. In the sentinel lymph-node, a subcapsular tumor embole of about 50 cancer cells was identified on the extemporaneous cryo-cut section, but not on further sections after paraffin-embedding of the sample. Considering this tumor metastatic potential, we decided to assess HER2 status on the metastatic embole using pathological and molecular micro-methods. We laser-microdissected the tumor cells, extracted their DNA, and performed droplet-digital-PCR (ddPCR) for HER2 gene copy number variation. The HER2/RNaseP allele ratio was 5.2 in the laser-microdissected tumor cells, similar to the 5.3 ratio in the HER2-overexpressing breast cancer cell line BT-474. We thus optimized the adjuvant treatment of our patient and she received a trastuzumab-based adjuvant chemotherapy. Competing Interests: CONFLICTS OF INTEREST The authors declare no competing interests.
Databáze:	MEDLINE
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