Development and validation of rapid and sensitive LC methods with PDA and fluorescence detection for determination of piribedil in rat plasma and brain tissues and their pharmacokinetic application.

Autor: Uppuluri CT; Department of Pharmacy, BITS-Pilani Hyderabad Campus, Hyderabad, Telangana, India., Dalvi AV; Department of Pharmacy, BITS-Pilani Hyderabad Campus, Hyderabad, Telangana, India., Bommireddy EP; Department of Pharmacy, BITS-Pilani Hyderabad Campus, Hyderabad, Telangana, India., Ravi PR; Department of Pharmacy, BITS-Pilani Hyderabad Campus, Hyderabad, Telangana, India.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2018 Oct; Vol. 32 (10), pp. e4303. Date of Electronic Publication: 2018 Jul 02.
DOI: 10.1002/bmc.4303
Abstrakt: Simple, selective and sensitive high-performance liquid chromatographic (HPLC) bioanalytical methods using fluorescence (FL) and photodiode array (PDA) detectors were developed and validated for determination of piribedil (PBD), an anti-Parkinson's drug, in rat plasma and brain samples, with telmisartan as internal standard (IS). Protein precipitation technique was used to extract PBD from both biological matrices. Chromatographic separation was achieved on a Phenomenex Kinetex C 18 end-capped column (250 × 4.6 mm, 5 μm), with 38:62 v/v acetonitrile and ammonium acetate buffer (pH 5.0) as mobile phase at 1.0 mL/min flow rate. Linear response in the concentration ranges 5-300 and 150-3000 ng/mL in plasma, and 15-900 and 450-9000 ng/g in brain tissue were achieved in FL and PDA detectors, respectively. The chromatograms were extracted at 239 nm in case of PDA detection and at excitation wavelength of 239 nm and emission wavelength of 385 nm in case of FL detection. FL detection was found to be more sensitive compared with PDA detection. The developed methods were successfully employed in determining the plasma time course, brain distribution and the pharmacokinetic parameters of PBD following intravenous bolus administration of the drug in male Wistar rats.
(© 2018 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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