The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station.

Autor: Grebennikova TV; Federal Research Center of Epidemiology and Microbiology named after Gamalei, Moscow, Russia.; Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow 117198, Russia., Syroeshkin AV; Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow 117198, Russia., Shubralova EV; Central Research Institute of Machine Building, Korolev, Russia., Eliseeva OV; Federal Research Center of Epidemiology and Microbiology named after Gamalei, Moscow, Russia., Kostina LV; Federal Research Center of Epidemiology and Microbiology named after Gamalei, Moscow, Russia., Kulikova NY; Federal Research Center of Epidemiology and Microbiology named after Gamalei, Moscow, Russia., Latyshev OE; Federal Research Center of Epidemiology and Microbiology named after Gamalei, Moscow, Russia., Morozova MA; Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow 117198, Russia., Yuzhakov AG; Federal Research Center of Epidemiology and Microbiology named after Gamalei, Moscow, Russia., Zlatskiy IA; Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow 117198, Russia.; Dumanskii Institute of Colloid and Water Chemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine., Chichaeva MA; Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow 117198, Russia., Tsygankov OS; Korolev Rocket and Space Corporation «Energia», Korolev, Russia.
Jazyk: angličtina
Zdroj: TheScientificWorldJournal [ScientificWorldJournal] 2018 Apr 18; Vol. 2018, pp. 7360147. Date of Electronic Publication: 2018 Apr 18 (Print Publication: 2018).
DOI: 10.1155/2018/7360147
Abstrakt: Cosmic dust samples from the surface of the illuminator of the International Space Station (ISS) were collected by a crew member during his spacewalk. The sampler with tampon in a vacuum container was delivered to the Earth. Washouts from the tampon's material and the tampon itself were analyzed for the presence of bacterial DNA by the method of nested PCR with primers specific to DNA of the genus Mycobacteria , DNA of the strains of capsular bacteria Bacillus , and DNA encoding 16S ribosomal RNA. The results of amplification followed by sequencing and phylogenetic analysis indicated the presence of the bacteria of the genus Mycobacteria and the extreme bacterium of the genus Delftia in the samples of cosmic dust. It was shown that the DNA sequence of one of the bacteria of the genus Mycobacteria was genetically similar to that previously observed in superficial micro layer at the Barents and Kara seas' coastal zones. The presence of the wild land and marine bacteria DNA on the ISS suggests their possible transfer from the stratosphere into the ionosphere with the ascending branch of the global electric circuit. Alternatively, the wild land and marine bacteria as well as the ISS bacteria may all have an ultimate space origin.
Databáze: MEDLINE
Externí odkaz: