Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization.

Autor: Svobodová K; 1 Laboratory of Environmental Biotechnology, Institute of Microbiology of the Czech Academy of Sciences , Prague, Czech Republic ., Semerád J; 1 Laboratory of Environmental Biotechnology, Institute of Microbiology of the Czech Academy of Sciences , Prague, Czech Republic .; 2 Faculty of Science, Institute for Environmental Studies, Charles University , Prague, Czech Republic ., Petráčková D; 3 Laboratory of Post-Transcriptional Control of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences , Prague, Czech Republic ., Novotný Č; 1 Laboratory of Environmental Biotechnology, Institute of Microbiology of the Czech Academy of Sciences , Prague, Czech Republic .
Jazyk: angličtina
Zdroj: Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2018 Jul/Aug; Vol. 24 (6), pp. 830-838. Date of Electronic Publication: 2018 May 30.
DOI: 10.1089/mdr.2017.0406
Abstrakt: Quantitative changes in antibiotic resistance genes (ARGs) were investigated in six urban wastewater treatment plants (WWTPs) treating municipal and industrial wastewaters. In a selected WWTP, the fate of ARGs was studied in a 1-year time interval and in two phases of wastewater treatment process. Nine ARGs (tetW, tetO, tetA, tetB, tetM, bla TEM , ermB, sul1, and intl1) were quantified in total and their relative abundance assessed by ARG copies/16SrRNA copies. From the tetracycline resistance genes, tetW was the only one detected in all sampled WWTPs. Its relative abundance in the nitrification tank of WWTP5 was found stable during the 1-year period, but was lowered by secondary sedimentation processes in the wastewater treatment down to 24% compared to the nitrification tank. Bacterial isolates showing high tetracycline resistance (minimal inhibition concentrations >100 μg/mL) were identified as members of Acinetobacter, Klebsiella, Citrobacter, Bacillus, and Enterobacter genera. Dynamic shifts in the relative abundance of ermB and sul1 were also demonstrated in wastewater samples from WWTP5.
Databáze: MEDLINE