Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart.

Autor:	Almeida CM; Universidade Estadual da Paraíba - UEPB, Department of Dentistry, Campina Grande, PB, Brasil., Lima RF; Universidade Estadual da Paraíba - UEPB, Department of Dentistry, Campina Grande, PB, Brasil., Costa TKVLD; Universidade Estadual da Paraíba - UEPB, Department of Dentistry, Campina Grande, PB, Brasil., Sousa IMO; Universidade Estadual de Campinas - Unicamp, Chemical, Biological and Agricultural Pluridisciplinary Research Center, Campinas, SP, Brazil., Cabral EC; Universidade Estadual de Campinas - Unicamp, Chemical, Biological and Agricultural Pluridisciplinary Research Center, Campinas, SP, Brazil., Basting RT; Universidade Estadual de Campinas - Unicamp, Chemical, Biological and Agricultural Pluridisciplinary Research Center, Campinas, SP, Brazil., Torre AD; Universidade Estadual de Campinas - Unicamp, Chemical, Biological and Agricultural Pluridisciplinary Research Center, Campinas, SP, Brazil., Cavalcanti YW; Universidade Federal da Paraíba - UFPB, Department of Dentistry, Campina Grande, PB, Brasil., Rosalen PL; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Department of Physiological Sciences, Piracicaba, SP, Brazil., Duarte MCT; Universidade Estadual de Campinas - Unicamp, Chemical, Biological and Agricultural Pluridisciplinary Research Center, Campinas, SP, Brazil., Ruiz ALTG; Universidade Estadual de Campinas - Unicamp, Chemical, Biological and Agricultural Pluridisciplinary Research Center, Campinas, SP, Brazil., Foglio MA; Universidade Estadual de Campinas - Unicamp, Chemical, Biological and Agricultural Pluridisciplinary Research Center, Campinas, SP, Brazil., Godoy GP; Universidade Estadual da Paraíba - UEPB, Department of Dentistry, Campina Grande, PB, Brasil., Costa EMMB; Universidade Estadual da Paraíba - UEPB, Department of Dentistry, Campina Grande, PB, Brasil.
Jazyk:	angličtina
Zdroj:	Brazilian oral research [Braz Oral Res] 2018 May 24; Vol. 32, pp. e41. Date of Electronic Publication: 2018 May 24.
DOI:	10.1590/1807-3107bor-2018.vol32.0041
Abstrakt:	The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line - both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines.
Databáze:	MEDLINE
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