A Cyclotide Isolated from Noisettia orchidiflora (Violaceae).
| Autor: | Bobey AF; NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil., Pinto MEF; NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil., Cilli EM; NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil., Lopes NP; NPPNS, Faculty of Pharmaceutical Sciences of Ribeirão Preto-USP, The University of São Paulo-USP, Ribeirão Preto-São Paulo, Brazil., Bolzani VDS; NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Planta medica [Planta Med] 2018 Aug; Vol. 84 (12-13), pp. 947-952. Date of Electronic Publication: 2018 May 29. |
| DOI: | 10.1055/a-0632-2204 |
| Abstrakt: | Biologically active cyclotides have been found on some flowering plants species and are involved in the role of the plant protection. As part of studies focusing on peptides from Brazilian plant species, we are reporting the detection by LC-MS of several cyclotides from leaves and stems of Noisettia orchidiflora (Violaceae). From stems it was possible to isolate and characterize a cyclotide named Nor A. Its primary structure (amino acid sequence) was established by MALDI-TOF-MS, based on the y- and b- type ion series, after reduction and alkylation reactions, as well as enzymatic digestion using the enzymes endoproteinase glutamic acid (endoGlu-C), trypsin, and chymotrypsin. Furthermore, the amino acid analysis was also described. Competing Interests: The authors declare no conflict of interest. (Georg Thieme Verlag KG Stuttgart · New York.) |
| Databáze: | MEDLINE |
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