Heterogeneity of adult masseter muscle satellite cells with cardiomyocyte differentiation potential.

Autor: Huang W; Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Liang J; Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Feng Y; Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Jia Z; Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Jiang L; Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Cai W; Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Paul C; Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Gu JG; Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Stambrook PJ; Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Millard RW; Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Zhu XL; Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China., Zhu P; Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. Electronic address: tanganqier@163.com., Wang Y; Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA. Electronic address: yi-gang.wang@uc.edu.
Jazyk: angličtina
Zdroj: Experimental cell research [Exp Cell Res] 2018 Oct 01; Vol. 371 (1), pp. 20-30. Date of Electronic Publication: 2018 May 26.
DOI: 10.1016/j.yexcr.2018.05.028
Abstrakt: Although resident cardiac stem cells have been reported, regeneration of functional cardiomyocytes (CMs) remains a challenge. The present study identifies an alternative progenitor source for CM regeneration without the need for genetic manipulation or invasive heart biopsy procedures. Unlike limb skeletal muscles, masseter muscles (MM) in the mouse head are developed from Nkx2-5 mesodermal progenitors. Adult masseter muscle satellite cells (MMSCs) display heterogeneity in developmental origin and cell phenotypes. The heterogeneous MMSCs that can be characterized by cell sorting based on stem cell antigen-1 (Sca1) show different lineage potential. While cardiogenic potential is preserved in Sca1 + MMSCs as shown by expression of cardiac progenitor genes (including Nkx2-5), skeletal myogenic capacity is maintained in Sca1 - MMSCs with Pax7 expression. Sca1 + MMSC-derived beating cells express cardiac genes and exhibit CM-like morphology. Electrophysiological properties of MMSC-derived CMs are demonstrated by calcium transients and action potentials. These findings show that MMSCs could serve as a novel cell source for cardiomyocyte replacement.
(Copyright © 2018. Published by Elsevier Inc.)
Databáze: MEDLINE
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