Clinical and microbiological parameters of naturally occurring periodontitis in the non-human primate Macaca mulatta .

Autor: Colombo APV; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Paster BJ; Departments of Microbiology and Applied Oral Sciences, Forsyth Institute, Cambridge, MA, USA., Grimaldi G; Department of Immunology, Fiocruz Primate Research Center, Rio de Janeiro, Brazil., Lourenço TGB; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Teva A; Department of Immunology, Fiocruz Primate Research Center, Rio de Janeiro, Brazil., Campos-Neto A; Departments of Microbiology and Applied Oral Sciences, Forsyth Institute, Cambridge, MA, USA., McCluskey J; Sanofi Pasteur, Cambridge, MA, USA., Kleanthous H; Sanofi Pasteur, Cambridge, MA, USA., Van Dyke TE; Departments of Microbiology and Applied Oral Sciences, Forsyth Institute, Cambridge, MA, USA., Stashenko P; Departments of Microbiology and Applied Oral Sciences, Forsyth Institute, Cambridge, MA, USA.
Jazyk: angličtina
Zdroj: Journal of oral microbiology [J Oral Microbiol] 2017 Nov 25; Vol. 9 (1), pp. 1403843. Date of Electronic Publication: 2017 Nov 25 (Print Publication: 2017).
DOI: 10.1080/20002297.2017.1403843
Abstrakt: Background : Non-human primates appear to represent the most faithful model of human disease, but to date the oral microbiome in macaques has not been fully characterized using next-generation sequencing. Objective : In the present study, we characterized the clinical and microbiological features of naturally occurring periodontitis in non-human primates ( Macaca mulatta ). Design : Clinical parameters of periodontitis including probing pocket depth (PD) and bleeding on probing (BOP) were measured in 40 adult macaques (7-22 yrs), at six sites per tooth. Subgingival plaque was collected from diseased and healthy sites, and subjected to 16S rDNA sequencing and identification at the species or higher taxon level. Results : All macaques had mild periodontitis at minimum, with numerous sites of PD ≥ 4 mm and BOP. A subset (14/40) had moderate-severe disease, with >2 sites with PD ≥ 5mm, deeper mean PD, and more BOP. Animals with mild vs moderate-severe disease were identical in age, suggesting genetic heterogeneity. 16S rDNA sequencing revealed that all macaques had species that were identical to those in humans or closely related to human counterparts, including Porphyromonas gingivalis which was present in all animals. Diseased and healthy sites harboured distinct microbiomes; however there were no significant differences in the microbiomes in moderate-severe vs. mild periodontitis. Conclusions : Naturally occurring periodontitis in older macaques closely resembles human adult periodontitis, thus validating a useful model to evaluate novel anti-microbial therapies.
Competing Interests: Drs J. McCluskey and H. Kleanthous are employees of Sanofi-Pasteur. No other conflicts of interest were reported by the authors.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje