Engineering PCL/lignin nanofibers as an antioxidant scaffold for the growth of neuron and Schwann cell.

Autor: Wang J; State Key Laboratory of Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Center for Nanofibers and Nanotechnology, E3-05-14, Department of Mechanical Engineering, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, 117576, Singapore., Tian L; Center for Nanofibers and Nanotechnology, E3-05-14, Department of Mechanical Engineering, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, 117576, Singapore., Luo B; Singapore Institute for Neurotechnology, National University of Singapore, 28 Medical Drive, #05-COR, 119077, Singapore., Ramakrishna S; Center for Nanofibers and Nanotechnology, E3-05-14, Department of Mechanical Engineering, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, 117576, Singapore., Kai D; Institute of Materials Research and Engineering (IMRE), A*STAR, 2 Fusionopolis Way. Innovis, #08-03, 138634, Singapore. Electronic address: kaid@imre.a-star.edu.sg., Loh XJ; Institute of Materials Research and Engineering (IMRE), A*STAR, 2 Fusionopolis Way. Innovis, #08-03, 138634, Singapore; Department of Materials Science and Engineering, National University of Singapore, 9 Engineering Drive 1, 117576, Singapore; Singapore Eye Research Institute, 11 Third Hospital Avenue, 168751, Singapore., Yang IH; Singapore Institute for Neurotechnology, National University of Singapore, 28 Medical Drive, #05-COR, 119077, Singapore., Deen GR; Soft Materials Laboratory, Natural Sciences and Science Education, National Institute of Education Nanyang Technological University, Singapore., Mo X; State Key Laboratory of Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. Electronic address: xmm@dhu.edu.cn.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2018 Sep 01; Vol. 169, pp. 356-365. Date of Electronic Publication: 2018 May 12.
DOI: 10.1016/j.colsurfb.2018.05.021
Abstrakt: Antioxidant is critical for the successful of nerve tissue regeneration, and biomaterials with antioxidant activity might be favorable for peripheral nerve repair. Lignin, a biopolymer from wood with excellent antioxidant properties, is still "unexplored" as biomaterials. To design an antioxidative bioscaffold for nerve regeneration, here we synthesized lignin-polycaprolactone (PCL) copolymers via solvent free ring-opening polymerization (ROP). Then such lignin-PCL copolymers were incorporated with PCL and engineered into nanofibrous scaffolds for supporting the growth of neuron and Schwann cell. Our results showed that the addition of lignin-PCL enhanced the mechanical properties of PCL nanofibers and endowed them with good antioxidant properties (up to 98.3 ± 1.9% free radical inhibition within 4 h). Cell proliferation assay showed that PCL/lignin-PCL nanofibers increased cell viability compared to PCL fibers, especially after an oxidative challenge. Moreover, Schwann cells and dorsal root ganglion (DRG) neurons cultured on the nanofibers to assess their potential for nerve regeneration. These results suggested that nanofibers with lignin copolymers promoted cell proliferation of both BMSCs and Schwann cells, enhanced myelin basic protein expressions of Schwann cells and stimulated neurite outgrowth of DRG neurons. In all, these sustainable, intrinsically antioxidant nanofibers may be a potential candidate for nerve TE applications.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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