Antimicrobial susceptibility of Clostridium difficile isolated from food and environmental sources in Western Australia.

Autor: Lim SC; School of Biomedical Sciences, The University of Western Australia, Nedlands, WA, Australia., Androga GO; School of Biomedical Sciences, The University of Western Australia, Nedlands, WA, Australia; Department of Microbiology, PathWest Laboratory Medicine, Nedlands, WA, Australia., Knight DR; School of Biomedical Sciences, The University of Western Australia, Nedlands, WA, Australia; School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, Australia., Moono P; School of Biomedical Sciences, The University of Western Australia, Nedlands, WA, Australia., Foster NF; OzFoodNet, Communicable Diseases Control Directorate, Department of Health, Government of Western Australia, Perth, WA, Australia., Riley TV; School of Biomedical Sciences, The University of Western Australia, Nedlands, WA, Australia; School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, Australia; OzFoodNet, Communicable Diseases Control Directorate, Department of Health, Government of Western Australia, Perth, WA, Australia; School of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia. Electronic address: thomas.riley@uwa.edu.au.
Jazyk: angličtina
Zdroj: International journal of antimicrobial agents [Int J Antimicrob Agents] 2018 Sep; Vol. 52 (3), pp. 411-415. Date of Electronic Publication: 2018 May 23.
DOI: 10.1016/j.ijantimicag.2018.05.013
Abstrakt: We recently reported a high prevalence of Clostridium difficile in retail vegetables, compost and lawn in Western Australia. The objective of this study was to investigate the antimicrobial susceptibility of previously isolated food and environmental C. difficile isolates from Western Australia. A total of 274 C. difficile isolates from vegetables, compost and lawn were tested for susceptibility to a panel of 10 antimicrobial agents (fidaxomicin, vancomycin, metronidazole, rifaximin, clindamycin, erythromycin, amoxicillin/clavulanic acid, moxifloxacin, meropenem and tetracycline) using the agar incorporation method. Fidaxomicin was the most potent agent (MIC 50 /MIC 90 , 0.06/0.12 mg/L). Resistance to fidaxomicin and metronidazole was not detected and resistance to vancomycin (0.7%) and moxifloxacin (0.7%) was low. However, 103 isolates (37.6%) showed resistance to at least one agent, and multidrug resistance was observed in 3.9% of the resistant isolates (4/103), all of which came from compost. A significantly greater proportion of compost isolates were resistant to clindamycin, erythromycin and tetracycline compared with food and/or lawn isolates. Clostridium difficile ribotype (RT) 014/020 showed greater clindamycin resistance than other less common RTs (P = 0.008, χ 2 ). Contaminated vegetables, compost and lawn could be playing an intermediary role in the transmission of C. difficile from animals to humans. Environmental strains of C. difficile could also function as a reservoir for antimicrobial resistance genes of clinical relevance. This study provides a baseline for future surveillance of antimicrobial resistance in environmental C. difficile isolates in Australia.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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