An RNA Origami Octahedron with Intrinsic siRNAs for Potent Gene Knockdown.

Autor: Høiberg HC; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus 8000 C, Denmark., Sparvath SM; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus 8000 C, Denmark., Andersen VL; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus 8000 C, Denmark.; Department of Molecular Biology and Genetics, Aarhus University, Aarhus 8000 C, Denmark., Kjems J; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus 8000 C, Denmark.; Department of Molecular Biology and Genetics, Aarhus University, Aarhus 8000 C, Denmark., Andersen ES; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus 8000 C, Denmark.; Department of Molecular Biology and Genetics, Aarhus University, Aarhus 8000 C, Denmark.
Jazyk: angličtina
Zdroj: Biotechnology journal [Biotechnol J] 2019 Jan; Vol. 14 (1), pp. e1700634. Date of Electronic Publication: 2018 Jun 13.
DOI: 10.1002/biot.201700634
Abstrakt: The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.
(© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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