| Autor: |
Guan C; College of Animal Science and Technology, China Agricultural University, No. 2 Yuan Mingyuan Xi Road, Beijing, 100193, China., Huang YH; College of Animal Science and Technology, China Agricultural University, No. 2 Yuan Mingyuan Xi Road, Beijing, 100193, China., Cui X; College of Animal Science and Technology, China Agricultural University, No. 2 Yuan Mingyuan Xi Road, Beijing, 100193, China., Liu SJ; College of Animal Science and Technology, China Agricultural University, No. 2 Yuan Mingyuan Xi Road, Beijing, 100193, China., Zhou YZ; College of Animal Science and Technology, China Agricultural University, No. 2 Yuan Mingyuan Xi Road, Beijing, 100193, China., Zhang YW; College of Animal Science and Technology, China Agricultural University, No. 2 Yuan Mingyuan Xi Road, Beijing, 100193, China. zywei@126.com.; Beijing Key Laboratory for Grassland Science, China Agricultural University, Beijing, China. zywei@126.com.; National Energy R&D Center for Biomass (NECB), Beijing, China. zywei@126.com. |
| Abstrakt: |
Key Message: Genetic improvement through overexpressing PuP5CS in switchgrass is feasible for enhancing plant salt stress tolerance. Switchgrass (Panicum virgatum L.) has developed into a dedicated bioenergy crop. To improve the biomass production of switchgrass grown on different types of soil, abiotic stress tolerance traits are considered for its genetic improvement. Proline accumulation is a widespread response when plants are subjected to abiotic stresses such as drought, cold and salinity. In plants, P5CS gene encodes the key regulatory enzyme that plays a crucial role in proline biosynthesis. Here, we introduced the PuP5CS gene (from Puccinellia chinampoensis) into switchgrass by Agrobacterium-mediated transformation. Transgenic lines overexpressing the PuP5CS gene showed phenotypic advantages, in leaf width, internode diameter, internode length, tiller numbers and precocious flowering under normal conditions, and the transgenic lines displayed better regenerative capacity in forming more tillers after harvest. Moreover, the PuP5CS gene enhanced the salt tolerance of transgenic switchgrass by altering a wide range of physiological responses. In accordance with the physiological results, histological analysis of cross sections through the leaf blade showed that the areas of bulliform cells and bundle sheath cells were significantly increased in PuP5CS-overexpressing leaves. The expression levels of ROS scavenging-associated genes in transgenic plants were higher than in control plants under salt stress. The results show that genetic improvement through overexpressing PuP5CS in switchgrass is feasible for enhancing plant stress tolerance. |
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