Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons.

Autor:	Li D; From the Department of Psychiatry, Yale University, New Haven, Connecticut 06520., Musante V; From the Department of Psychiatry, Yale University, New Haven, Connecticut 06520., Zhou W; From the Department of Psychiatry, Yale University, New Haven, Connecticut 06520., Picciotto MR; From the Department of Psychiatry, Yale University, New Haven, Connecticut 06520., Nairn AC; From the Department of Psychiatry, Yale University, New Haven, Connecticut 06520 angus.nairn@yale.edu.
Jazyk:	angličtina
Zdroj:	The Journal of biological chemistry [J Biol Chem] 2018 Jul 13; Vol. 293 (28), pp. 11179-11194. Date of Electronic Publication: 2018 May 25.
DOI:	10.1074/jbc.RA117.001519
Abstrakt:	Striatin-1, a subunit of the serine/threonine phosphatase PP2A, is preferentially expressed in neurons in the striatum. As a member of the striatin family of B subunits, striatin-1 is a core component together with PP2A of a multiprotein complex called STRIPAK, the striatin-interacting phosphatase and kinase complex. Little is known about the function of striatin-1 or the STRIPAK complex in the mammalian striatum. Here, we identify a selective role for striatin-1 in striatal neuron maturation. Using a small hairpin RNA (shRNA) knockdown approach in primary striatal neuronal cultures, we determined that reduced expression of striatin-1 results in increased dendritic complexity and an increased density of dendritic spines, classified as stubby spines. The dendritic phenotype was rescued by co-expression of a striatin-1 mutant construct insensitive to the knockdown shRNA but was not rescued by co-expression of PP2A- or Mob3-binding deficient striatin-1 constructs. Reduction of striatin-1 did not result in deficits in neuronal connectivity in this knockdown model, as we observed no abnormalities in synapse formation or in spontaneous excitatory postsynaptic currents. Thus, this study suggests that striatin-1 is a regulator of neuronal development in striatal neurons. (© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.)
Databáze:	MEDLINE
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