Metabolomic analysis of heat-hardening in adult green-lipped mussel (Perna canaliculus): A key role for succinic acid and the GABAergic synapse pathway.

Autor: Dunphy BJ; School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address: b.dunphy@auckland.ac.nz., Ruggiero K; Department of Statistics, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand., Zamora LN; Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand., Ragg NLC; Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand.
Jazyk: angličtina
Zdroj: Journal of thermal biology [J Therm Biol] 2018 May; Vol. 74, pp. 37-46. Date of Electronic Publication: 2018 Mar 13.
DOI: 10.1016/j.jtherbio.2018.03.006
Abstrakt: We evaluated the thermotolerance (LT 50 ) of adult green-lipped mussels (Perna canaliculus) following an acute thermal challenge in the summer of 2012 and the winter of 2013. Mussels were grouped into two treatments, naïve (N, no prior heat treatment) and heat-hardened (HH = 1 h at 29 °C, 12 h recovery at ambient) before being immersed for 3 h in water of varying temperature, i.e. Ambient (Control), 25, 29, 31, 33, and 35 °C with subsequent mortality monitored for 30 days. As expected, naïve mussels were less thermotolerant than heat-hardened i.e. Summer LT 50 , N = 31.9, HH = 33.5 °C; Winter LT 50 , N = 31.4, HH = 33.8 °C. Moreover, at 33 °C no heat-hardened mussels died compared to 100% mortality in naïve specimens. At 35 °C all mussels died regardless of treatment. For the 'Summer' mussels, metabolite abundances in gill tissues of both naïve and heat-hardened mussels were quantified. For mussels at 33 °C, succinic acid was significantly higher in naïve mussels than heat-hardened mussels, indicating perturbations to mitochondrial pathways in these thermally stressed mussels. Additionally, analysis of biochemical pathway activity suggested a loss of neural control i.e. significantly reduced GABAergic synapse activity, in naïve vs. heat-hardened mussels at 33 °C. Taken together these findings suggest that heat-hardening improves mussel survival at higher temperatures by delaying the onset of cellular anaerobic metabolism, and by maintaining inhibition of neural pathways. Such results offer new perspectives on the complex suite of sub-cellular stress responses operating within thermally stressed organisms.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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