Recapitulating and Deciphering Human Pancreas Development From Human Pluripotent Stem Cells in a Dish.
| Autor: | Petersen MBK; Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), University of Copenhagen, Copenhagen, Denmark; Department of Islet and Stem Cell Biology, Novo Nordisk, Måløv, Denmark., Gonçalves CAC; Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), University of Copenhagen, Copenhagen, Denmark., Kim YH; Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), University of Copenhagen, Copenhagen, Denmark. Electronic address: yung.kim@sund.ku.dk., Grapin-Botton A; Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), University of Copenhagen, Copenhagen, Denmark. Electronic address: anne.grapin-botton@sund.ku.dk. |
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| Jazyk: | angličtina |
| Zdroj: | Current topics in developmental biology [Curr Top Dev Biol] 2018; Vol. 129, pp. 143-190. Date of Electronic Publication: 2018 May 08. |
| DOI: | 10.1016/bs.ctdb.2018.02.009 |
| Abstrakt: | Here, we review how human pluripotent stem cell models of pancreas development have emerged and became an important tool to study human development and disease. Initially developed toward the production of β cells for diabetes therapy, the protocols have been refined based on knowledge of pancreas development in model organisms. While the cells produced are closer and closer to the end goal of a functional β cell, these models have also been used to carry out functional experiments addressing gene function and expression as well as regulatory and epigenetic landscape changes during human pancreas development. They thereby complement model organisms and reports from human genetic variants predisposing to different forms of diabetes, as well as observations on human fetal tissue. In this review, we therefore compare these different sources of information and discuss how human stem cell models are evolving to inform us on pancreatic diseases and possible treatments. (© 2018 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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